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Adjuvant-free immunization with hemagglutinin-Fc fusion proteins as an approach to influenza vaccines

Adjuvant-free immunization with hemagglutinin-Fc fusion proteins as an approach to influenza vaccines

Loureiro, Silvia, Ren, Junyuan, Phapugrangkul, Pongsathon, Colaco, Camilo A., Bailey, Christopher R., Shelton, Holly, Molesti, Eleonora, Temperton, Nigel J., Barclay, Wendy S. and Jones, Ian M. (2010) Adjuvant-free immunization with hemagglutinin-Fc fusion proteins as an approach to influenza vaccines. Journal of Virology, 85 (6). pp. 3010-3014. ISSN 0022-538X (Print), 1098-5514 (Online) (doi:10.1128/​JVI.01241-10)

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Abstract

The hemagglutinins (HAs) of human H1 and H3 influenza viruses and avian H5 influenza virus were produced as recombinant fusion proteins with the human immunoglobulin Fc domain. Recombinant HA-human immunoglobulin Fc domain (HA-HuFc) proteins were secreted from baculovirus-infected insect cells as glycosylated oligomer HAs of the anticipated molecular mass, agglutinated red blood cells, were purified on protein A, and were used to immunize mice in the absence of adjuvant. Immunogenicity was demonstrated for all subtypes, with the serum samples demonstrating subtype-specific hemagglutination inhibition, epitope specificity similar to that seen with virus infection, and neutralization. HuFctagged HAs are potential candidates for gene-to-vaccine approaches to influenza vaccination.

Item Type: Article
Additional Information: [1] Published ahead of print on 29 December 2010. [2] The authors have paid a fee to allow immediate free access to this article. [3] Citation: Silvia Loureiro, Junyuan Ren, Pongsathon Phapugrangkul, Camilo A. Colaco, Christopher R. Bailey, Holly Shelton, Eleonora Molesti, Nigel J. Temperton, Wendy S. Barclay, Ian M. Jones. J. Virol. 2011, 85(6):3010. DOI: 10.1128/JVI.01241-10.
Uncontrolled Keywords: virus infection, gene-to-vaccine, influenza vaccination
Subjects: R Medicine > RC Internal medicine
R Medicine > RZ Other systems of medicine
Faculty / Department / Research Group: Faculty of Engineering & Science > Medway School of Pharmacy
Related URLs:
Last Modified: 03 May 2016 05:06
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/8549

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