In vitro characterisation of chitosan based xerogels for potential buccal delivery of proteins
Ayensu, Isaac, Mitchell, John C. and Boateng, Joshua S. (2012) In vitro characterisation of chitosan based xerogels for potential buccal delivery of proteins. Carbohydrate Polymers, 89 (3). pp. 935-941. ISSN 0144-8617 (doi:10.1016/j.carbpol.2012.04.039)Full text not available from this repository.
Chitosan and thiolated-chitosan based xerogels have been prepared by lyophilising aqueous gels of the polymers incorporating glycerol, d-mannitol and BSA with an annealing process. Analytical characterisation was by circular dichroism, infrared spectroscopy, X-ray diffraction and scanning electron microscopy. Swelling capacities of 1110 ± 23.3% and 480 ± 18.2% were obtained for the chitosan and TG-chitosan xerogels respectively. Thiolation caused improved in vitro mucoadhesive properties by demonstrating peak adhesive force of 4.5 ± 0.7 and 5.8 ± 0.2 N, and total work of adhesion of 6.5 ± 1.0 and 19 ± 0.8 mJ for chitosan and thiolated-chitosan xerogels respectively. In vitro drug dissolution studies using Bradford's assay showed BSA release of 91.5 ± 3.7% and 94.4 ± 7.3% from the chitosan and thiolated-chitosan xerogels respectively. These results demonstrate the potential of developing lyophilised thiolated-chitosan xerogels with optimised mucoadhesion characteristics for buccal mucosa delivery of proteinbased drugs.
|Uncontrolled Keywords:||thiolated-chitosan, lyophilisation, mucoadhesion, buccal delivery, protein|
|Subjects:||Q Science > QD Chemistry|
R Medicine > R Medicine (General)
|School / Department / Research Groups:||School of Science|
School of Science > Department of Pharmaceutical, Chemical & Environmental Sciences
|Last Modified:||20 Jan 2014 10:55|
Actions (login required)