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Wheat blast: histopathology and transcriptome reprogramming in response to adapted and nonadapted Magnaporthe isolates

Wheat blast: histopathology and transcriptome reprogramming in response to adapted and nonadapted Magnaporthe isolates

Tufan, Hale A., McGrann, Graham R. D., Magusin, Andreas, Morel, Jean-Benoit, Miche, Lucie and Boyd, Lesley A. (2009) Wheat blast: histopathology and transcriptome reprogramming in response to adapted and nonadapted Magnaporthe isolates. New Phytologist, 184 (2). pp. 473-484. ISSN 1469-8137 (on-line) (doi:10.1111/j.1469-8137.2009.02970.x)

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Abstract

Blast disease (causal agent Magnaporthe oryzae) has presented as a new and serious field disease of wheat in South America. Here, we investigated the responses of wheat to both adapted and nonadapted isolates of the blast fungus Magnaporthe, examining cellular defence and transcriptional changes.

Resistance towards the nonadapted isolate was associated with the formation of appositions, here termed halos, beneath attempted Magnaporthe grisea penetration sites that wheat-adapted, M. oryzae isolates were able to breach.

Transcriptome analysis indicated extensive transcriptional reprogramming following inoculation with both wheat-adapted and nonadapted isolates of Magnaporthe. Functional annotation of many of the differentially expressed transcripts classified into the categories: cell rescue and defence, plant metabolism, cellular transport and regulation of transcription (although a significant number of transcripts remain unclassified).

Defence-related transcripts induced in common by adapted and nonadapted isolates were differentially regulated in response to M. oryzae and M. grisea isolates over time. Differential expression of genes involved in cellular transport indicated the importance of this process in plant defence. Functional characterisation of these transcripts and their role in defence may eventually lead to the identification of broad-spectrum resistance mechanisms in wheat towards Magnaporthe.

Item Type: Article
Additional Information: [1] MIAME/Plant-compliant microarray data from this study have been deposited in PLEXdb (http://www.plexdb.org) with the accession number TA24
Uncontrolled Keywords: defence response, nonhost resistance, plant–pathogen interactions, transcription profiling, wheat blast
Subjects: Q Science > QK Botany
Faculty / Department / Research Group: Faculty of Engineering & Science > Natural Resources Institute
Related URLs:
Last Modified: 18 Oct 2016 09:13
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/8208

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