Propagation of Ptilostemon chamaepeuce (L.) less through tissue culture

Antonidaki-Giatromanolaki, Anna, Orchard, John, Papadimitriou, Michel, Dragassaki, Magdalena and Vlahos, Ioannis (2010) Propagation of Ptilostemon chamaepeuce (L.) less through tissue culture. Journal of Biological Research-Thessaloniki, 13. pp. 105-111. ISSN 1790-045 X (print)

Abstract

Ptilostemon chamaepeuce, a plant native to Crete, has the potential to develop into a new flori-cultural crop; therefore regeneration via organogenesis was studied. Seeds were germinated at rates higher than 84% to produce plant material for in vitro culture. The influence of growth regulators was investigated on MS and WPM media supplemented with 0, 1, 2 or 4 mg l-1 BA and 0, 0.5, 1, or 2 mg l-1 NAA and their combinations. BA at high concentrations (2 or 4 mg l-1) increased the number of shoots (7.6 shoots per explant), while longer shoots were recorded in media without growth regulators. Shoot proliferation was similar in both MS and WPM media. However, chlorosis and dryness of leaves were observed on WPM. Regenerated shoots rooted after culture in MS medium with 0.5, 1 or 2 mg l-1 NAA and 1, 2, or 4 mg l-1 IBA at rates higher than 80%. No rooting was observed in media without auxins. Rooted cuttings were easily acclimatized (90% survival) in polystyrene plugs at low intensity light and were successfully established in pots under greenhouse conditions.

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