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Assessment of fluidity of different invasomes by electron spin resonance and differential scanning calorimetry

Assessment of fluidity of different invasomes by electron spin resonance and differential scanning calorimetry

Dragicevic-Curic, Nina, Friedrich, Manfred, Petersen, Silvia, Scheglmann, Dietrich, Douroumis, Dennis, Plass, Winfried and Fahr, Alfred (2011) Assessment of fluidity of different invasomes by electron spin resonance and differential scanning calorimetry. International Journal of Pharmaceutics, 412 (1-2). pp. 85-94. ISSN 0378-5173

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Abstract

The aim of this study was to investigate the influence of membrane-softening components (terpenes/terpene mixtures, ethanol) on fluidity of phospholipid membranes in invasomes, which contain besides phosphatidylcholine and water, also ethanol and terpenes. Also mTHPC was incorporated into invasomes in order to study its molecular interaction with phospholipids in vesicular membranes. Fluidity of bilayers was investigated by electron spin resonance (ESR) using spin labels 5- and 16-doxyl stearic acid and by differential scanning calorimetry (DSC). Addition of 1% of a single terpene/terpene mixture led to significant fluidity increase around the C16 atom of phospholipid acyl chains comprising the vesicles. However, it was not possible to differentiate between the influences of single terpenes or terpene mixtures. Incorporation of mTHPC into the bilayer of vesicles decreased fluidity near the C16 atom of acyl chains, indicating its localization in the inner hydrophobic zone of bilayers. These results are in agreement with DSC measurements, which showed that terpenes increased fluidity of bilayers, while mTHPC decreased fluidity. Thus, invasomes represent vesicles with very high membrane fluidity. However, no direct correlation between fluidity of invasomes and their penetration enhancing ability was found, indicating that besides fluidity also other phenomena might be responsible for improved skin delivery of mTHPC.

Item Type: Article
Uncontrolled Keywords: liposomes, invasomes, fluidity, electron spin resonance, differential scanning calorimetry, temoporfin
Subjects: Q Science > Q Science (General)
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Pharmaceutical, Chemical & Environmental Sciences
Related URLs:
Last Modified: 07 Dec 2016 11:16
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/7499

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