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Development and physico-mechanical characterisation of lyophilised chitosan wafers as potential protein drug delivery systems via the buccal mucosa

Development and physico-mechanical characterisation of lyophilised chitosan wafers as potential protein drug delivery systems via the buccal mucosa

Ayensu, Isaac, Mitchell, John C. and Boateng, Joshua S. (2011) Development and physico-mechanical characterisation of lyophilised chitosan wafers as potential protein drug delivery systems via the buccal mucosa. Colloids and Surfaces B: Biointerfaces, 91. pp. 258-265. ISSN 0927-7765 (doi:10.1016/j.colsurfb.2011.11.004)

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Abstract

Lyophilised wafers from chitosan have been developed as potential protein drug delivery systems via the buccal mucosa. Wafers were prepared by lyophilising aqueous gels of the polymer incorporating varying concentrations of glycerol as plasticizer and d-mannitol as cryoprotectant. The different formulations were characterised by their physico-mechanical properties in order to select the optimum system for further development. The optimised formulation with 6.5 mg each of both plasticizer and cryoprotectant was loaded with bovine serum albumin and lyophilised with or without annealing. Differential scanning calorimetry was used to determine the appropriate lyophilisation cycle by evaluating thermal events before lyophilisation and possible phase separation of bovine serum albumin after lyophilisation. Texture analysis was employed to investigate the in vitro mucoadhesive properties in tensile mode, residual moisture content by thermo-gravimetric analysis while hydration capacity and drug release studies were performed in 0.1 M phosphate buffered saline. Microscopic architecture and crystallinity were examined using scanning electron microscopy and X-ray diffractometry respectively. The ease of hydration, in vitro mucoadhesive characteristics, microscopic architecture and BSA release were influenced by the annealing process. A 7 h cumulative percentage drug release of 91.5% and 80.1% was observed for the annealed and non-annealed wafers respectively. The results showed the potential of employing lyophilised chitosan wafers for buccal mucosa delivery of protein based drugs.

Item Type: Article
Additional Information: [1] Available online 15 November 2011. [2] Published in print: 1 March 2012. [3] Published in Colloids and Surfaces B: Biointerfaces, (2012), Volume 91, pp. 258–265.
Uncontrolled Keywords: chitosan, lyophilised wafers, mucoadhesion, buccal delivery, protein
Subjects: R Medicine > RS Pharmacy and materia medica
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Pharmaceutical, Chemical & Environmental Sciences
Related URLs:
Last Modified: 17 Oct 2016 09:11
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/7180

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