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Host range, purification, and genetic variability in Sweet potato chlorotic fleck virus

Host range, purification, and genetic variability in Sweet potato chlorotic fleck virus

Aritua, V., Barg, E., Adipala, E., Gibson, R.W., Lesemann, D.E. and Vetten, H.J. (2009) Host range, purification, and genetic variability in Sweet potato chlorotic fleck virus. Plant Disease, 93 (1). pp. 87-93. ISSN 0191-2917 (doi:10.1094/PDIS-93-1-0087)

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Abstract

Sweet potato chlorotic fleck virus (SPCFV) has recently been classified as a putative new member of the genus Carlavirus (family Flexiviridae) on the basis of its molecular properties. In this study, SPCFV was characterized in terms of host range, physical and biological characteristics, and genetic variability. In addition to sweet potato, SPCFV infected some plant species in the families Convolvulaceae, Chenopodiaceae, and Solanaceae. Limited numbers of virus particles were observed in the assimilation parenchyma cells of infected plant tissues; some cells had a distorted and enlarged endoplasmic reticulum though without any cytoplasmic and amorphous inclusions. The normal length of SPCFV particles was determined to be approximately 800 nm. In enzyme-linked immunosorbent assays, polyclonal antibodies raised against purified SPCFV virions were able to detect the virus in infected sweet potato and indicator plant tissues. In immunoelectron microscopy, SPCFV particles were all strongly decorated when reacted with homologous antiserum. Comparison of the 3′ terminal part of the genome of a range of geographically diverse isolates revealed a high level of genetic diversity. The amino acid sequence identity in the coat protein and the nucleic acid binding protein ranged from 89 to 99.7% and from 75.9 to 99.2%, respectively. Phylogenetic analysis of both proteins showed a geographically associated clustering into two genogroups.

Item Type: Article
Additional Information: [1] Sequences published in this article have been assigned GenBank/EMBL/DDBJ database accession numbers EU375897 to 375910. [2] This is an Open Access article. The APS (American Phytopathological Society) Council makes journal research freely available online after 12 months. Research published in ... Plant Disease ... is freely accessible after 12 months of subscriber-only access. In addition, authors have the opportunity to purchase immediate open access to journal articles. Single articles with open access are clearly marked in the journal tables of contents.
Uncontrolled Keywords: carlavirus, sweet potato, Uganda, Africa
Subjects: Q Science > QK Botany
S Agriculture > SB Plant culture
Faculty / Department / Research Group: Faculty of Engineering & Science > Natural Resources Institute
Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Related URLs:
Last Modified: 12 May 2016 17:47
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/6060

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