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Determination of Michaelis-Menten parameters obtained from isothermal flow calorimetric data

Determination of Michaelis-Menten parameters obtained from isothermal flow calorimetric data

O'Neill, M.A.A., Beezer, A.E., Mitchell, J.C. ORCID: 0000-0003-2945-3292, Orchard, J. and Connor, J.A. (2004) Determination of Michaelis-Menten parameters obtained from isothermal flow calorimetric data. Thermochimica Acta, 417 (2). pp. 187-192. ISSN 0040-6031 (doi:10.1016/j.tca.2003.07.019)

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Abstract

Recent papers have reported [Thermochim. Acta 399 (2003) 63; Thermochim. Acta, in press] the results of a preliminary inter/intra laboratory study into the suitability of the base-catalysed hydrolysis of methyl paraben as a test and reference reaction for isothermal flow-through calorimeters. It was shown that this reaction can be used to investigate the flow characteristics of the instrument being used. It has also allowed, for the first time, the calculation of accurate values for the rate constant and for the enthalpy change, DeltaH (hereafter H (enthalpy) for simplicity) of reaction directly from the calorimetric data, free from assumption. These findings have been extended to permit the direct determination of Michaelis-Menten based kinetic parameters from calorimetric data again free from assumption (except that the system conforms to Michaelis-Menten kinetic theory). This paper describes the method used for such an analysis and reports the results of a preliminary study on the urea/urease enzymatic system.

Item Type: Article
Additional Information: [1] First available online: 27 February 2004. [2] Honour Issue on the Retirement of Prof A. E. Beezer.
Uncontrolled Keywords: Michaelis-Menten parameters, isothermal flow, calorimetric data, flow calorimetry, enzymes, Michaelis-Menten kinetics
Subjects: Q Science > QD Chemistry
R Medicine > RS Pharmacy and materia medica
Pre-2014 Departments: School of Science
School of Science > Medway Sciences Research Group
Related URLs:
Last Modified: 14 Oct 2016 09:11
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/4186

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