Characterisation of pumpkin yellow vein mosaic virus from India

Muniyappa, V., Maruthi, M.N., Babitha, C.R., Colvin, John, Briddon, R.W. and Rangaswamy, K.T. (2003) Characterisation of pumpkin yellow vein mosaic virus from India. Annals of Applied Biology, Volume 142 Issue 3, Pages 323 - 331 (3). pp. 323-331. ISSN 0003-4746

Abstract

Yellow vein mosaic disease symptoms occur frequently in pumpkin in India. Diseased plants show
vein yellowing, which sometimes coalesces to form chlorotic patches. Infected plants are stunted and
flowers drop prematurely, greatly reducing yields. Diseased plants are infected by a begomovirus,
designated pumpkin yellow vein mosaic virus (PYVMV), which is transmitted readily and in a persistent
manner by the whitefly, Bemisia tabaci. Transmission of PYVMV requires minimum acquisition and
inoculation access periods of 30 min and 10 min, respectively. The minimum latent period in the insect
is 6 h and the virus persists in the vector for at least 8 days. PYVMV has a narrow host range consisting
of a small number of cucurbit species and some tobacco cultivars. It was detected serologically in
diseased plants and in viruliferous B. tabaci using polyclonal antibodies in a double-antibody sandwich
enzyme-linked immunosorbent assay. Reactions with monoclonal antibodies in a triple-antibody
sandwich ELISA showed that PYVMV has an epitope profile distinct from those of other begomoviruses
from the Indian sub-continent. Polymerase chain reaction amplified fragments from the putative viral
coat and movement protein genes. Based on comparative phylogeny of complete coat protein gene
sequences, PYVMV was most similar to

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