Host range, vector relationships and sequence comparison of a begomovirus infecting hibiscus in India

Rajeshwari, R., Chowda, Reddy R.V., Maruthi, M.N., Colvin, John, Seal, Susan and Muniyappa, V. (2005) Host range, vector relationships and sequence comparison of a begomovirus infecting hibiscus in India. Annals of Applied Biology, 147 (1). pp. 15-25. ISSN 0003-4746

Abstract

Hibiscus leaf curl disease (HLCuD) occurs widely in India. Infected hibiscus
plants show vein thickening, upward curling of leaves and enations on
the abaxial leaf surface, reduction in leaf size and stunting. The commonlyoccurring
weeds (Ageratum conyzoides, Croton bonplandianum and Euphorbia
geniculata), Nicotiana benthamiana, Nicotiana glutinosa and Nicotiana tabacum
(var. Samsun, Xanthi), cotton and tomato were shown to be susceptible to
HLCuD. One of the four species of hibiscus (Hibiscus rosa-sinensis) and 75 of
the 101 commercial hybrids/varieties grown in the Bangalore area of southern
India were also susceptible. Two virus isolates associated with HLCuD
from Bangalore, South India (Ban), and Bhubaneswar, North India (Bhu),
were detected serologically and by PCR-mediated amplification of virus
genomes. The isolates were characterised by sequencing a fragment of DNA-A
component (1288 nucleotides) and an associated satellite DNA molecule of
682 nucleotides. Phylogenetic analyses of these DNA-A sequences clustered
them with Old World cotton-infecting begomoviruses and closest to Cotton leaf
curl Multan virus (CLCuMV) at 95–97% DNA-A nucleotide identities. The
682-nucleotide satellite DNA molecules associated with the HLCuD samples
Ban and Bhu shared 96.9% sequence identity with each other and maximum
identity (93.1–93.9% over positions 158–682) with ;1350-nucleotide DNA-b
satellite molecules associated with cotton leaf curl disease in Pakistan and
India (accession nos AJ298903, AJ316038). HLCuD in India, therefore,
appears to be associated with strains of CLCuMV, a cotton-infecting
begomovirus from Pakistan, which is transmitted in a persistent manner by
Bemisia tabaci

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