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Induction of apoptosis in host cells: a survival mechanism for Leishmania parasites?

Induction of apoptosis in host cells: a survival mechanism for Leishmania parasites?

Getti, G. T., Cheke, R. A. and Humber, D. P. (2008) Induction of apoptosis in host cells: a survival mechanism for Leishmania parasites? Parasitology, 135 (12). pp. 1391-1399. ISSN 0031-1820 (doi:10.1017/S0031182008004915)

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Abstract

Leishmania parasites invade host macrophages, causing infections that are either limited to skin or spread to internal organs. In this study, 3 species causing cutaneous leishmaniasis, L. major, L. aethiopica and L. tropica, were tested for their ability to interfere with apoptosis in host macrophages in 2 different lines of human monocyte-derived macrophages (cell lines THP-1 and U937) and the results confirmed in peripheral blood mononuclear cells (PBMC). All 3 species induced early apoptosis 48 h after infection (expression of phosphatidyl serine on the outer membrane). There were significant increases in the percentage of apoptotic cells both for U937 and PBMC following infection with each of the 3 species. Early apoptotic events were confirmed by mitochondrial membrane permeabilization detection and caspase activation 48 and 72 h after infection. Moreover, the percentage of infected THP-1 and U937 macrophages increased significantly (up to 100%) following treatment with an apoptosis inducer. Since phosphatidyl serine externalization on apoptosing cells acts as a signal for engulfment by macrophages, induction of apoptosis in the parasitized cells could actively participate in spreading the infection. In summary, parasite-containing apoptotic bodies with intact membranes could be released and phagocytosed by uninfected macrophages.

Item Type: Article
Additional Information: [1] Copyright 2008 Cambridge University Press. [2] The definitive version of this paper - G.T. GETTI, R.A. CHEKE and D.P. HUMBER (2008). Induction of apoptosis in host cells: a survival mechanism for Leishmania parasites?. Parasitology, 135 , pp 1391-1399. doi:10.1017/S0031182008004915.
Uncontrolled Keywords: Leishmania, THP-1, U937, infection, apoptosis
Subjects: R Medicine > RC Internal medicine
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Pharmaceutical, Chemical & Environmental Sciences
Faculty of Engineering & Science > Natural Resources Institute
Faculty of Engineering & Science > Natural Resources Institute > Pest Behaviour Research Group
Related URLs:
Last Modified: 09 Dec 2016 11:22
URI: http://gala.gre.ac.uk/id/eprint/2194

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