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Prooxidant action of knipholone anthrone: Copper dependent reactive oxygen species generation and DNA damage

Prooxidant action of knipholone anthrone: Copper dependent reactive oxygen species generation and DNA damage

Habtemariam, S. and Dagne, E. (2009) Prooxidant action of knipholone anthrone: Copper dependent reactive oxygen species generation and DNA damage. Food and Chemical Toxicology, 47 (7). pp. 1490-1494. ISSN 0278-6915 (doi:10.1016/j.fct.2009.03.032)

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Abstract

Knipholone (KP) and knipholone anthrone (KA) are natural 4-phenylanthraquinone structural analogues with established differential biological activities including in vitro antioxidant and cytotoxic properties. By using DNA damage as an experimental model, the comparative Cu(II)-dependent prooxidant action of these two compounds were studied. In the presence of Cu(II) ions, the antioxidant KA (3.1-200 [mu]M) but not KP (6-384 [mu]M) caused a concentration-dependent pBR322 plasmid DNA strand scission. The DNA damage induced by KA could be abolished by reactive oxygen species scavengers, glutathione and catalase as well as EDTA and a specific Cu(I) chelator bathocuproine disulfonic acid. In addition to Cu(II) chelating activity, KA readily reduces Cu(II) to Cu(I). Copper-dependent generation of reactive oxygen species and the subsequent macromolecular damage may be involved in the antimicrobial and cytotoxic activity of KA.

Item Type: Article
Uncontrolled Keywords: knipholone, knipholone anthrone, copper, reactive oxygen species, DNA damage
Subjects: Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Pharmaceutical, Chemical & Environmental Sciences
Related URLs:
Last Modified: 08 Dec 2016 15:27
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/2058

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