Skip navigation

Circular dichroism studies of low molecular weight hydrogelators: the use of SRCD and addressing practical issues

Circular dichroism studies of low molecular weight hydrogelators: the use of SRCD and addressing practical issues

Sitsanidis, Efstatios D., Piras, Carmen C., Alexander, Bruce, Siligardi, Giuliano, Javorfi, Tamas, Hall, Andrew J. and Edwards, Alison (2018) Circular dichroism studies of low molecular weight hydrogelators: the use of SRCD and addressing practical issues. Chirality. ISSN 0899-0042 (Print), 1520-636X (Online) (In Press) (doi:10.1002/chir.22850)

[img] PDF (Author Accepted Manuscript)
19260 ALEXANDER_Circular_Dichroism_Studies_of_Low_Molecular_Weight_Hydrogelators_2018.pdf - Accepted Version
Restricted to Repository staff only

Download (1MB)

Abstract

Circular dichroism (CD) spectroscopy has been used extensively for the investigation of the conformation and configuration of chiral molecules, but its use for evaluating the mode of self-assembly in soft materials has been limited. Herein, we report a protocol for the study of such materials by electronic circular dichroism (CD) spectroscopy using commercial/benchtop instruments and synchrotron radiation (SR) using the B23 beamline available at Diamond Light Source. The use of B23 beamtime for SRCD was advantageous due to the unique enhanced spatial resolution achieved because of its highly collimated and small beamlight cross-section (ca 250 μm) and higher photon flux in the far UV region (175-250 nm) enhancing the signal-to-noise ratio relative to benchtop CD instruments. A set of low molecular weight (LMW) hydrogelators, comprising two Fmoc-protected enantiomeric monosaccharides and one Fmoc-dipeptide (Fmoc-FF) were studied. The research focused on the optimization of sample preparation and handling, which then enabled the characterisation of sample conformational homogeneity and thermal stability. CD spectroscopy, in combination with other spectroscopic techniques and microscopy, will allow a better insight into the self-assembly of chiral building blocks into higher order structural architectures.

Item Type: Article
Uncontrolled Keywords: gels, materials, circular dichroism, spectroscopy
Subjects: Q Science > QC Physics
Q Science > QD Chemistry
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Department of Pharmaceutical, Chemical & Environmental Sciences
Last Modified: 03 May 2018 10:00
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: GREAT a
URI: http://gala.gre.ac.uk/id/eprint/19260

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics