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Preparation and evaluation of streptomycin and diclofenac loaded mucoadhesive films for wound healing

Preparation and evaluation of streptomycin and diclofenac loaded mucoadhesive films for wound healing

Pawar, H. V., Tetteh, J. and Boateng, J. ORCID: 0000-0002-6310-729X (2012) Preparation and evaluation of streptomycin and diclofenac loaded mucoadhesive films for wound healing. Wound Repair and Regeneration, 20 (2). A34. ISSN 1067-1927 (Print), 1524-475X (Online) (doi:10.1111/j.1524-475X.2012.00774.x)

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Abstract

Solvent cast films combining natural and synthetic polymers for maximum healing effects for wound healing have been prepared. Streptomycin (anti-microbial) was combined with
diclofenac (anti-inflammatory) was used for synergistic effect. Films of polyox® WSR301 (POL) and carrageenan (CAR) in different ratios and plasticised with glycerol were prepared by solvent casting. The films were physically characterised by swelling and hydration capacity, scanning electron microscopy (SEM), differential scanning calorimetry (DSC), texture analysis for mechanical properties and in vitro mucoadhesive properties. In vitro antibacterial studies using disk diffusion test and drug release profiles with Franz diffusion cell were conducted. SEM studies of unplasticized blank films showed presence of spherulitic crystals of POL with homogeneous interpenetrating polymer network. Both blank (BLK) and drug loaded (DL) films showed homogeneous morphology. DSC results of pure streptomycin and diclofenac showed a melt peak at 146.77°C and 293.96°C respectively, however this peak was absent in DL films indicating molecular dispersion of the drug. Films containing 25% w/w glycerol exhibited significantly (n=3, P<0.001) high work of adhesion, stickiness and cohesiveness and high hydration and swelling index corresponding to unplasticized films. BLK films containing glycerol showed higher tensile strength (TS) (1.1-12.3 MPa) than DL films (0.7-9.5 MPa). Film plasticized with 25 % w/w glycerol (1.00 N/mm2 TS, 1031% elongation at break) revealed soft and tough formulation. Antibacterial studies showed that diclofenac and streptomycin significantly (n=3, P<0.01) increased zone of inhibition (ZOI) in Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa in contrast to their individual ZOIs. Unplasticized films showed significantly (n=2 P<0.0318) higher cumulative % drug release of streptomycin and diclofenac (60.07±1.56%, 63.39±1.92% respectively.) compared to plasticized films (52.11±1.34%, 55.26±2.25%) within 72 hrs. Films prepared from POL and CAR containing streptomycin and diclofenac, have potential to prevent and
treat wound infections and targets inflammatory phase of wound healing to relieve pain.

Item Type: Article
Additional Information: [1] This is an abstract of a paper presented at the 22nd Annual Meeting of the Wound Healing Society, 6th Joint Meeting with the European Tissue Repair Society, in conjunction with the Symposium on Advanced Wound Care (SAWC-Spring/WHS Joint Meeting). The 22nd Annual Meeting was held from 19-22 April 2012 in Atlanta, Georgia. [2] Wound Repair and Regeneration is published on behalf of the Wound Healing Society.
Uncontrolled Keywords: polymer blend, solvent cast films, wound healing, mucoadhesion, antibacterial, in vitro drug release
Subjects: R Medicine > RM Therapeutics. Pharmacology
R Medicine > RS Pharmacy and materia medica
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Pharmaceutical, Chemical & Environmental Sciences
Related URLs:
Last Modified: 06 Dec 2016 15:48
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/10674

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