APCs, BCA, CD,Enteric, GST–GFP, IPTG, immobilisation, LP, MALDI-TOF, MDA, PP, Peyer’s patch, SANS, SDS PAGE, silica, synthetic protein, VDS, vaccine, antigen presenting cells, bicinchoninic acid assay, circular dichroism, glutathione-S-transferase (GST), lamina propria, maternally derived antibodies, matrix assisted laser desorption ionisation time o, small angle neutron scattering, sodium dodecyl sulphate polyacrylamide gel electro, vaccine delivery systemsenteric, immobilisation

Pettit, Marie, Dyer, Paul D.R., Mitchell, John C. ORCID: 0000-0003-2945-3292 , Griffiths, Peter C. ORCID: 0000-0002-6686-1271 , Alexander, Bruce, Cattoz, Beatrice, Heenan, Richard K., King, Stephen M., Schweins, Ralf, Pullen, Frank, Wicks, Stephen R. and Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 (2014) Construction and physiochemical characterisation of a multi-composite, potential oral vaccine delivery system (VDS). International Journal of Pharmaceutics, 468 (1-2). pp. 264-271. ISSN 0378-5173 (doi:https://doi.org/10.1016/j.ijpharm.2014.03.046)

Antisense; Anthrax toxin; siRNA; Endocytosis; Cell biology; RNAi; PEG-dilemma; Non-viral

Dyer, Paul D.R., Shepherd, Thomas R., Gollings, Alexander S., Shorter, Susan A., Gorringe-Pattrick, Monique A. M., Tang, Chun-Kit, Cattoz, Beatrice N., Baillie, Les, Griffiths, Peter C. ORCID: 0000-0002-6686-1271 and Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 (2015) Disarmed anthrax toxin delivers antisense oligonucleotides and siRNA with high efficiency and low toxicity. Journal of Controlled Release, 220 (A). pp. 316-328. ISSN 0168-3659 (doi:https://doi.org/10.1016/j.jconrel.2015.10.054)

Antisense; Drug Delivery; Endocytosis; Gene Therapy; siRNA; Toxin

Shorter, Susan, Gollings, Alexander, Gorringe-Pattrick, Monique, Coakley, Emma, Dyer, Paul D.R. and Richardson, Simon ORCID: 0000-0002-7927-0649 (2016) The potential of toxin-based drug delivery systems for enhanced nucleic acid therapeutic delivery. Expert Opinion on Drug Delivery, 14 (5). pp. 685-696. ISSN 1742-5247 (Print), 1744-7593 (Online) (doi:https://doi.org/10.1080/17425247.2016.1227781)

GFP, Marker, Endocytosis, Protein stability, Protease, Drug delivery, Membrane

Shorter, Susan, Pettit, Marie W., Dyer, Paul D.R., Coakley-Youngs, J. Emma, Gorringe-Pattrick, Monique A. M., El-Daher, Samer and Richardson, Simon ORCID: 0000-0002-7927-0649 (2017) Green Fluorescent Protein (GFP): is seeing believing and is that enough? Journal of Drug Targeting, 25 (9-10). pp. 809-817. ISSN 1061-186X (Print), 1029-2330 (Online) (doi:https://doi.org/10.1080/1061186X.2017.1358725)

In vitro model, Spreading, Leishmania aethiopica, Leishmania mexicana, Human monocytes, Infection, THP-1, Retinoic acid

Rai, Rajeev, Dyer, Paul, Richardson, Simon ORCID: 0000-0002-7927-0649 , Harbige, Laurence and Getti, Giulia ORCID: 0000-0003-1402-8496 (2017) Apoptotic induction induces Leishmania aethiopica and L. mexicana spreading in terminally differentiated THP-1 cells. Parasitology, 144 (14). pp. 1912-1921. ISSN 0031-1820 (Print), 1469-8161 (Online) (doi:https://doi.org/10.1017/S0031182017001366)

Ricin toxin; Endocytosis; Polyphenol; Epigallocatechin gallate; eGCG; Tea

Dyer, Paul D. R., Kotha, Arun K., Gollings, Alex S., Shorter, Susan A., Shepherd, Thomas R., Pettit, Marie W., Alexander, Bruce D., Getti, Giulia T. M. ORCID: 0000-0003-1402-8496 , El-Daher, Samer, Baillie, Les and Richardson, Simon C. W. ORCID: 0000-0002-7927-0649 (2016) An in vitro evaluation of epigallocatechin gallate (eGCG) as a biocompatible inhibitor of ricin toxin. Biochimica et Biophysica Acta (BBA) - General Subjects, 1860 (7). pp. 1541-1550. ISSN 0304-4165 (doi:https://doi.org/10.1016/j.bbagen.2016.03.024)

antisense oligonucleotide; ASO; cytosolic delivery

Richardson, Dyer, Mitchell (2014) WO 2014203008 A1 Antisense oligonucleotide compositions. WO2014203008 A1.

endocytosis, drug delivery, organelle, fluorescent microscopy, live-cell imaging, polymers

Dyer, Paul D.R., Kotha, Arun K., Pettit, Marie W. and Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 (2013) Imaging select mammalian organelles using fluorescent microscopy: application to drug delivery. In: Weissig, Volkmar, Elbayoumi, Tamer and Olsen, Mark, (eds.) Methods in Molecular Biology: Cellular and subcellular nanotechnology. Methods in Molecular Biology, 991 . Humana Press, New York, USA, pp. 195-210. ISBN 9781627033350 (doi:https://doi.org/10.1007/978-1-62703-336-7)

endocytosis, endomembrane, poly(amidoamine), toxin, trafficking

Dyer, Paul D.R. and Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 (2011) Delivery of biologics to select organelles – the role of biologically active polymers. Expert Opinion on Drug Delivery, 8 (4). pp. 403-407. ISSN 1742-5247 (doi:https://doi.org/10.1517/17425247.2011.558080)

gene delivery, layer-by-layer assembly, nanoparticles, self-assembly, zeta-potential

Griffiths, Peter C. ORCID: 0000-0002-6686-1271 , Mauro, Nicolo, Murphy, Damien M., Carter, Emma, Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 , Dyer, Paul and Ferruti, Paolo (2013) Self-assembled PAA-based nanoparticles as potential gene and protein delivery systems. Macromolecular Bioscience, 13 (5). pp. 641-649. ISSN 1616-5187 (Print), 1616-5195 (Online) (doi:https://doi.org/10.1002/mabi.201200462)

peripheral blood-derived leukocytes, ex vivo, L. aethiopicaGFP, L. mexicanaGFP, CD4+, naïve, activated, effector, regulatory T cells

Ranatunga, Medhavi, Deacon, Andrew D. ORCID: 0000-0001-7630-4973 , Harbige, Laurence S., Dyer, Paul, Boateng, Joshua ORCID: 0000-0002-6310-729X and Getti, Giulia ORCID: 0000-0003-1402-8496 (2024) Ex vivo analysis of the association of GFP-expressing L. aethiopica and L. mexicana with human Peripheral Blood-Derived (PBD) leukocytes over 24 Hours. Microorganisms, 12 (9):1909. ISSN 2076-2607 (Online) (doi:https://doi.org/10.3390/microorganisms12091909)