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Ion mobility spectrometry-mass spectrometry (IMS-MS) of small molecules: separating and assigning structures to ions

Ion mobility spectrometry-mass spectrometry (IMS-MS) of small molecules: separating and assigning structures to ions

Lapthorn, Cris, Pullen, Frank and Chowdhry, Babur Z. (2013) Ion mobility spectrometry-mass spectrometry (IMS-MS) of small molecules: separating and assigning structures to ions. Mass Spectrometry Reviews, 32 (1). pp. 43-71. ISSN 0277-7037 (Print), 1098-2787 (Online) (doi:

(item_9541)_LAPTHORN_PULLEN_CHOWDHRY_authorarchive_PAPER1_Jul03_2012.pdf - Accepted Version
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The phenomenon of ion mobility (IM), the movement/transport of charged particles under the influence of an electric field, was first observed in the early 20th Century and harnessed later in ion mobility spectrometry (IMS). There have been rapid advances in instrumental design, experimental methods, and theory together with contributions from computational chemistry and gas-phase ion chemistry, which have diversified the range of potential applications of contemporary IMS techniques. Whilst IMS-mass spectrometry (IMS-MS) has recently been recognized for having significant research/applied industrial potential and encompasses multi-/cross-disciplinary areas of science, the applications and impact from decades of research are only now beginning to be utilized for "small molecule" species. This review focuses on the application of IMS-MS to "small molecule" species typically used in drug discovery (100-500 Da) including an assessment of the limitations and possibilities of the technique. Potential future developments in instrumental design, experimental methods, and applications are addressed. The typical application of IMS-MS in relation to small molecules has been to separate species in fairly uniform molecular classes such as mixture analysis, including metabolites. Separation of similar species has historically been challenging using IMS as the resolving power, R, has been low (3-100) and the differences in collision cross-sections that could be measured have been relatively small, so instrument and method development has often focused on increasing resolving power. However, IMS-MS has a range of other potential applications that are examined in this review where it displays unique advantages, including: determination of small molecule structure from drift time, "small molecule" separation in achiral and chiral mixtures, improvement in selectivity, identification of carbohydrate isomers, metabonomics, and for understanding the size and shape of small molecules. This review provides a broad but selective overview of current literature, concentrating on IMS-MS, not solely IMS, and small molecule applications. © 2012 Wiley Periodicals, Inc.

Item Type: Article
Additional Information: [1] This is the peer reviewed version of the following article: C. Lapthorn, F. Pullen, and B. Z. Chowdhry, “Ion mobility spectrometry-mass spectrometry (IMS-MS) of small molecules: Separating and assigning structures to ions,” Mass Spectrometry Reviews, vol. 32, no. 1, pp. 43–71, 2013 which has been published in final form at This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Uncontrolled Keywords: ion-mobility mass spectrometry, ion mobility spectrometry; mass spectrometry, small molecule, mass–mobility correlation, collision cross-section, ion mobility, FAIMS, drift-time, traveling wave, structural, computational, differential mobility spectrometry, differential mobility analyzer
Subjects: Q Science > QD Chemistry
Pre-2014 Departments: School of Science
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Last Modified: 26 Oct 2016 10:39

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