Li, He, Feng, Dongrui, wei, Sen, Deng, Xiaoyao, Dong, Huixun, Zhu, Aihua and Zhou, Shaobo ORCID: https://orcid.org/0000-0001-5214-2973 (2026) Bovine milk fat globule membrane-derived antioxidant peptides: enzymatic optimization, peptidomic profiling, and cytoprotective evaluation. Food Bioscience:109645. ISSN 2212-4292 (Print), 2212-4306 (Online) (doi:10.1016/j.fbio.2026.109645)

PDF (Author's Accepted Manuscript) 53997 ZHOU_Bovine_Milk_Fat_Globule_Membrane-derived_Antioxidant_Peptides_(AAM)_2026.pdf - Accepted Version Restricted to Repository staff only until 1 August 2027. Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (1MB) | Request a copy

Abstract

Milk fat globule membrane (MFGM) proteins possess diverse functional properties, however, the bioactivity of their derived peptides remains largely unexplored. In this study, MFGM was hydrolyzed using single and combined protease systems, pepsin-trypsin hydrolysis was identified as the optimal approach for promoting L6 cell viability. Response surface methodology optimized the hydrolysis conditions to 44.0°C, 100 min, and 5000 U/g, under which cell viability reached 134.12 ± 0.78%. LC-ESI-MS/MS peptidomic analysis identified 474 MFGM-derived peptides (MFGMP) enriched in short and low-molecular-weight sequences. Multi-criteria screening prioritized seven candidate peptides, with ARF emerging as a previously unrecognized MFGMP showing the strongest predicted SOD-binding affinity (−6.8 kcal/mol). Moreover, MFGMP alleviated dexamethasone (Dex)-induced oxidative injury by reducing reactive oxygen species accumulation and increasing SOD activity, while ARF further decreased reactive oxygen species by 18.8% and increased SOD activity by 7.3%. The present findings demonstrate that low-molecular-weight MFGMP, particularly ARF, a newly validated antioxidant peptide, protect L6 cells via Akt-NRF2signaling, highlighting their high-value utilization as natural antioxidants for functional food development.

Item Type:	Article
Uncontrolled Keywords:	MFGM, antioxidant peptides, enzymatic hydrolysis, peptidomics, cytoprotection
Subjects:	S Agriculture > S Agriculture (General) S Agriculture > SF Animal culture
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > School of Science (SCI)
Last Modified:	03 Aug 2026 14:20
URI:	https://gala.gre.ac.uk/id/eprint/53997

Actions (login required)

View Item

Downloads