Skip navigation

Expression and localization of AβPP in SH-SY5Y cells depends on differentiation state

Expression and localization of AβPP in SH-SY5Y cells depends on differentiation state

Riegerova, Petra, Brejcha, Jundrich, Bezdekova, Dagmar, Chum, Tomas, Masinova, Eva, Cermakova, Nikola, Ovsepian, Saak, Cebecauer, Marek and Stefl, Marek (2021) Expression and localization of AβPP in SH-SY5Y cells depends on differentiation state. Journal of Alzheimer's Disease, 82. pp. 485-491. ISSN 2542-4823 (Online) (doi:https://doi.org/10.3233/JAD-201409)

[img]
Preview
PDF
34380_Ovsepian_Expression_and_localisation_of_ABPP_in-SH-SY5Y_Cells_Depends_on.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial.

Download (656kB) | Preview

Abstract

Neuroblastoma cell line SH-SY5Y, due to its capacity to differentiate into neurons, easy handling, and low cost, is a common experimental model to study molecular events leading to Alzheimer's disease (AD). However, it is prevalently used in its undifferentiated state, which does not resemble neurons affected by the disease. Here, we show that the expression and localization of amyloid-β protein precursor (AβPP), one of the key molecules involved in AD pathogenesis, is dramatically altered in SH-SY5Y cells fully differentiated by combined treatment with retinoic acid and BDNF. We show that insufficient differentiation of SH-SY5Y cells results in AβPP mislocalization.

Item Type: Article
Uncontrolled Keywords: AbetaPP; Alzheimer’s disease; Differentiation; SH-SY5Y; Super-resolution microscopy
Subjects: Q Science > Q Science (General)
Faculty / School / Research Centre / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > School of Science (SCI)
Related URLs:
Last Modified: 15 Nov 2021 13:50
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
Selected for REF2021: None
URI: http://gala.gre.ac.uk/id/eprint/34380

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics