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PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava

PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava

Kavil, Siji, Otti, Gerald, Armitage, Andrew ORCID: 0000-0002-0610-763X, Bouvaine, Sophie ORCID: 0000-0002-0788-3243 and Maruthi, Midatharahally Narasegowda ORCID: 0000-0002-8060-866X (2021) PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava. Virology Journal, 18:184. ISSN 1743-422X (Print), 1743-422X (Online) (doi:https://doi.org/10.1186/s12985-021-01649-2)

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Abstract

Background: The phenylalanine ammonia lyase genes play crucial role in plant response to biotic and abiotic stresses. In this study, we characterized the role of PAL genes in increasing resistance to the Cassava brown streak virus that causes the economically important cassava brown streak disease (CBSD) on cassava in Africa. Methods: The whole transcriptomes of eight cassava varieties differing in resistance to CBSD were obtained at 1, 5 and 8 weeks after CBSV infection. Results: Analysis of RNA-Seq data identified the overexpression of PAL1, PAL2, cinnamic acid and two chalcone synthase genes in CBSD-resistant cassava varieties, which was subsequently confirmed by RT-qPCR. The exogenous application of Acibenzolar-S-Methyl induced PAL1 gene expression to enhance resistance in the susceptible var. Kalawe. In contrast, the silencing of PAL1 by RNA interference led to increased susceptibility of the resistant var. Kaleso to CBSD. Conclusions: PAL1 gene of the phenylpropanoid pathway has a major role in inducing resistance to CBSD in cassava plants and its early induction is key for CBSD resistance.

Item Type: Article
Uncontrolled Keywords: Cassava, CBSD, PAL1, resistance, induction
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Faculty / School / Research Centre / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Natural Resources Institute
Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Related URLs:
Last Modified: 29 Sep 2021 08:28
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
Selected for REF2021: None
URI: http://gala.gre.ac.uk/id/eprint/33981

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