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Identification of whitefly (Bemisia tabaci) proteins interacting with Tomato leaf curl Bangalore virus coat protein gene using Y2H system

Identification of whitefly (Bemisia tabaci) proteins interacting with Tomato leaf curl Bangalore virus coat protein gene using Y2H system

Prasannakumar, N R and Maruthi, Midatharahally Narasegowda ORCID: 0000-0002-8060-866X (2021) Identification of whitefly (Bemisia tabaci) proteins interacting with Tomato leaf curl Bangalore virus coat protein gene using Y2H system. International Journal of Tropical Insect Science. ISSN 1742-7584 (Print), 1742-7592 (Online) (doi:https://doi.org/10.1007/s42690-021-00561-0)

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Abstract

Tomato leaf curl Bangalore virus (ToLCBV) (Geminiviridae) causes the economically important tomato leaf curl virus disease (ToLCVD), and is transmitted by the whitefy, Bemisia tabaci (Gennadius) (Aleyrodidae: Hemiptera). Successful transmission of the virus by the insect requires safe translocation of the virus through diferent barriers inside the vector. During the translocation, the viral coat protein will interact with many whitefy proteins, while a few of them are benefcial to virus (e.g. heat shock proteins) but some may be harmful (e.g. whitefy immune proteins). We carried out the yeast two hybrid (Y2H) assays to identify the proteins of the B.tabaci Asia 1 genetic group interacting with ToLCBV coat protein (CP). The Y2H assay initially identifed a total of 425 putative interacting whitefy proteins on a low stringent selection media, and they were reduced to 324 when the yeast colonies were grown on a high stringent media, and of which about 274 colonies produced single bands in colony PCR experiments while the remaining colonies produced multiple bands. Further, high selection pressure assays confrmed a total of 102 whitefy proteins interacting with ToLCBV CP and these included the heat shock proteins (HSPs) 70 kDa, GroEL, nucleoproteins, vitellogenins, apolipophorins, 40 s ribosomal proteins, sorbitol dehydrogenase, dipeptidyl peptidase, E3 ubiquitin, annexin, GTP cyclohydrolase, tropomyosin, salivary secreted proteins, succinate dehydrogenase, lachesins, enolase and others. The identifed proteins could be potential targets for novel whitefy control strategies such as using RNAi or insecticide target sites for developing future disease and whitefy control strategies.

Item Type: Article
Uncontrolled Keywords: whitefly cDNA library, yeast two hybrid assay, gemini virus coat protein
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Faculty / School / Research Centre / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Natural Resources Institute
Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Related URLs:
Last Modified: 01 Dec 2021 01:38
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
Selected for REF2021: None
URI: http://gala.gre.ac.uk/id/eprint/33979

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