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Bemisia tabaci vesicle-associated membrane protein 2 interacts with begomoviruses and plays a role in virus acquisition

Bemisia tabaci vesicle-associated membrane protein 2 interacts with begomoviruses and plays a role in virus acquisition

Fan, Yun yun, Zhong, Yu-Wei, Zhao, Jing, Chi, Yao, Bouvaine, Sophie ORCID: 0000-0002-0788-3243, Liu, Shu-Sheng, Seal, Susan E. ORCID: 0000-0002-3952-1562 and Wang, Xiao-Wei (2021) Bemisia tabaci vesicle-associated membrane protein 2 interacts with begomoviruses and plays a role in virus acquisition. Cells, 10 (7):1700. ISSN 2073-4409 (Online) (doi:https://doi.org/10.3390/cells10071700)

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Abstract

Begomoviruses cause substantial losses to agricultural production, especially in tropical and subtropical regions, and are exclusively transmitted by members of the whitefly Bemisia tabaci species complex. However, the molecular mechanisms underlying the transmission of begomoviruses by their whitefly vector are not clear. In this study, we found that B. tabaci vesicle-associated membrane protein 2 (BtVAMP2) interacts with the coat protein (CP) of tomato yellow leaf curl virus (TYLCV), an emergent begomovirus that seriously impacts tomato production globally. After infection with TYLCV, the transcription of BtVAMP2 was increased. When the BtVAMP2 protein was blocked by feeding with a specific BtVAMP2 antibody, the quantity of TYLCV in B. tabaci whole body was significantly reduced. BtVAMP2 was found to be conserved among the B. tabaci species complex and also interacts with the CP of Sri Lankan cassava mosaic virus (SLCMV). When feeding with BtVAMP2 antibody, the acquisition quantity of SLCMV in whitefly whole body was also decreased significantly. Overall, our results demonstrate that BtVAMP2 interacts with the CP of begomoviruses and promotes their acquisition by whitefly

Item Type: Article
Additional Information: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Uncontrolled Keywords: acquisition; begomovirus; coat proteins; SLCMV; TYLCV; transmission; VAMP2; whiteflyacquisition; begomovirus; coat proteins; SLCMV; TYLCV; transmission; VAMP2; whitefly
Subjects: S Agriculture > S Agriculture (General)
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Natural Resources Institute
Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Last Modified: 24 Jul 2021 22:43
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
Selected for REF2021: None
URI: http://gala.gre.ac.uk/id/eprint/33403

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