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Identification and expression of "Secreted In Xylem" pathogenicity genes in fusarium oxysporum f. sp. pisi

Identification and expression of "Secreted In Xylem" pathogenicity genes in fusarium oxysporum f. sp. pisi

Jenkins, Sascha, Taylor, Andrew, Jackson, Alison C., Armitage, Andrew D. ORCID: 0000-0002-0610-763X, Bates, Helen J., Mead, Andrew, Harrison, Richard J. and Clarkson, John P. (2021) Identification and expression of "Secreted In Xylem" pathogenicity genes in fusarium oxysporum f. sp. pisi. Frontiers in Microbiology, 12:593140. ISSN 1664-302X (Online) (doi:https://doi.org/10.3389/fmicb.2021.593140)

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Abstract

Fusarium oxysporum is a soilborne fungal plant pathogen responsible for causing disease in many economically important crops with “special forms” (formae speciales) adapted to infect specific plant hosts. F. oxysporum f. sp. pisi (FOP) is the causal agent of Fusarium wilt disease of pea. It has been reported in every country where peas are grown commercially. Disease is generally controlled using resistant cultivars possessing single major gene resistance and therefore there is a constant risk of breakdown. The main aim of this work was to characterise F. oxysporum isolates collected from diseased peas in the United Kingdom as well as FOP isolates obtained from other researchers representing different races through sequencing of a housekeeping gene and the presence of Secreted In Xylem (SIX) genes, which have previously been associated with pathogenicity in other F. oxysporum f. spp. F. oxysporum isolates from diseased United Kingdom pea plants possessed none or just one or two known SIX genes with no consistent pattern of presence/absence, leading to the conclusion that they were foot-rot causing isolates rather than FOP. In contrast, FOP isolates had different complements of SIX genes with all those identified as race 1 containing SIX1, SIX6, SIX7, SIX9, SIX10, SIX11, SIX12, and SIX14. FOP isolates that were identified as belonging to race 2 through testing on differential pea cultivars, contained either SIX1, SIX6, SIX9, SIX13, SIX14 or SIX1, SIX6, SIX13. Significant upregulation of SIX genes was also observed in planta over the early stages of infection by different FOP races in pea roots. Race specific SIX gene profiling may therefore provide potential targets for molecular identification of FOP races but further research is needed to determine whether variation in complement of SIX genes in FOP race 2 isolates results in differences in virulence across a broader set of pea differential cultivars.

Item Type: Article
Additional Information: © 2021 Jenkins, Taylor, Jackson, Armitage, Bates, Mead, Harrison and Clarkson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Uncontrolled Keywords: Fusarium oxysporum f. sp. pisi, Pea, Fusarium wilt, formae speciales, race, Secreted In Xylem, gene expression, qPCR
Subjects: S Agriculture > S Agriculture (General)
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Natural Resources Institute
Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Last Modified: 25 Jun 2021 18:19
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
Selected for REF2021: None
URI: http://gala.gre.ac.uk/id/eprint/33160

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