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De novo transcriptomic analyses revealed some detoxification genes and related pathways responsive to noposion yihaogong® 5% EC (Lambda-Cyhalothrin 5%) exposure in spodoptera frugiperda third-instar larvae

De novo transcriptomic analyses revealed some detoxification genes and related pathways responsive to noposion yihaogong® 5% EC (Lambda-Cyhalothrin 5%) exposure in spodoptera frugiperda third-instar larvae

Hafeez, Muhammad, Li, Xiaowei, Zhang, Zhijun, Huang, Jun, Wang, Likun, Zhang, Jinming, Shah, Sakhawat, Khan, Muhammad Musa, Xu, Fei, Fernandez-Grandon, G. Mandela ORCID: 0000-0002-2993-390X, Zalucki, Myron P. and Lu, Yaobin (2021) De novo transcriptomic analyses revealed some detoxification genes and related pathways responsive to noposion yihaogong® 5% EC (Lambda-Cyhalothrin 5%) exposure in spodoptera frugiperda third-instar larvae. Insects, 12 (2):132. ISSN 2075-4450 (Online) (doi:https://doi.org/10.3390/insects12020132)

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Abstract

The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a polyphagous, invasive insect pest which causes significant losses in important crops wherever it has spread. The use of pesticides in agriculture is a key tool in the management of many important crop pests, including S. frugiperda, but continued use of insecticides has selected for various types of resistance, including enzyme systems that provide enhanced mechanisms of detoxification. In the present study, we analyzed the de novo transcriptome of S. frugiperda larvae exposed to Noposion Yihaogong® 5% emulsifiable concentrate (EC) insecticide focusing on detoxification genes and related pathways. Results showed that a total of 1819 differentially expressed genes (DEGs) were identified in larvae after being treated with Noposion Yihaogong® 5% EC insecticide, of which 863 were up- and 956 down-regulated. Majority of these differentially expressed genes were identified in numerous Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including metabolism of xenobiotics and drug metabolism. Furthermore, many of S. frugiperda genes involved in detoxification pathways influenced by lambda-cyhalothrin stress support their predicted role by further co-expression network analysis. Our RT-qPCR results were consistent with the DEG’s data of transcriptome analysis. The comprehensive transcriptome sequence resource attained through this study enriches the genomic platform of S. frugiperda, and the identified DEGs may enable greater molecular underpinnings behind the insecticide-resistance mechanism caused by lambda-cyhalothrin.

Item Type: Article
Uncontrolled Keywords: spodoptera, transcriptome analysis, detoxification pathways
Subjects: S Agriculture > S Agriculture (General)
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Natural Resources Institute
Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Faculty of Engineering & Science > Natural Resources Institute > Chemical Ecology Research Group
Last Modified: 12 Mar 2021 11:11
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
Selected for REF2021: None
URI: http://gala.gre.ac.uk/id/eprint/31181

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