Tembo, Mathias, Adediji, Adedapo O., Bouvaine, Sophie ORCID: 0000-0002-0788-3243, Chikoti, Patrick C., Seal, Susan ORCID: 0000-0002-3952-1562 and Silva, Gonҫalo ORCID: 0000-0001-5544-2947 (2020) A quick and sensitive diagnostic tool for detection of Maize streak virus. Scientific Reports, 10:19633. ISSN 2045-2322 (Online) (doi:https://doi.org/10.1038/s41598-020-76612-2)

Preview

PDF (Publisher's PDF - Open Access) 30221 SILVA_A_Quick_and_Sensitive_Diagnostic_Tool_(OA)_2020.pdf - Published Version Available under License Creative Commons Attribution. Download (1MB) | Preview

Abstract

Maize streak virus disease (MSVD), caused by Maize streak virus (MSV; genus Mastrevirus), is one of the most severe and widespread viral diseases that adversely reduces maize yield and threatens food security in Africa. An effective control and management of MSVD requires robust and sensitive diagnostic tests capable of rapid detection of MSV. In this study, a loop-mediated isothermal amplification (LAMP) assay was designed for the specific detection of MSV. This test has shown to be highly specific and reproducible and able to detect MSV in as little as 10 fg/µl of purified genomic DNA obtained from a MSV-infected maize plant, a sensitivity 105 times higher to that obtained with polymerase chain reaction (PCR) in current general use. The high degree of sequence identity between Zambian and other African MSV isolates indicate that this LAMP assay can be used for detecting MSV in maize samples from any region in Africa. Furthermore, this assay can be adopted in minimally equipped laboratories and with potential use in plant clinic laboratories across Africa strengthening diagnostic capacity in countries dealing with MSD.

Item Type:	Article
Additional Information:	© The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Uncontrolled Keywords:	MSV; Maize streak virus; diagnostics; LAMP; Food security; Capacity building
Subjects:	S Agriculture > S Agriculture (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department Faculty of Engineering & Science > Natural Resources Institute > Molecular Virology and Entomology Research Group
Last Modified:	16 Nov 2020 23:57
URI:	http://gala.gre.ac.uk/id/eprint/30221

Actions (login required)

View Item

Downloads