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Confocal imaging to reveal the microstructure of soybean processing materials

Confocal imaging to reveal the microstructure of soybean processing materials

Preece, Katherine E., Drost, Ellen, Hooshyar, Nasim, Krijgsman, Ardjan, Cox, Philip W. ORCID: 0000-0003-4683-0627 and Zuidam, Nicolaas J. (2014) Confocal imaging to reveal the microstructure of soybean processing materials. Journal of Food Engineering, 147. pp. 8-13. ISSN 0260-8774 (doi:

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Sustainable production of food products for human consumption is required to reduce negative impacts on the environment and to consumer’s health. Soybeans are an excellent source of nutritive plant proteins; aqueous extraction yields part of the available oil and protein from the legume. Many studies have been conducted which detail the various processing parameters and their effects on the extraction yields, yet there is little data on the localisation of nutritive components such as oil and protein in the fibrous unextracted by-product. Here we show a novel confocal laser scanning microscopy investigation of soybean processing materials and the physical effects of thermal treatment on the materials microstructure upon aqueous extraction. Various features, more specifically oil, protein (including protein aggregation) and cell wall structures, are visualised in the fibrous by-product, soy slurry and soy extract, with their presence both in the continuous phase and within intact cotyledon cells. Thermal treatment reduced the protein extraction yield; this is shown to be a result of aggregated protein bodies in the continuous phase and within intact cotyledons cells. Knowledge of the processing material microstructures can be applied to improve extraction yields and reduce waste production.

Item Type: Article
Additional Information: © 2014 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://
Uncontrolled Keywords: Aqueous extraction; Confocal laser scanning microscopy; Soy proteins; Sustainability
Subjects: T Technology > TA Engineering (General). Civil engineering (General)
Faculty / School / Research Centre / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > School of Engineering (ENG)
Last Modified: 12 Jan 2021 23:51

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