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Bioprinting and preliminary testing of highly reproducible novel bioink for potential skin regeneration

Bioprinting and preliminary testing of highly reproducible novel bioink for potential skin regeneration

Hafezi, Forough, Shorter, Susan, Tabriz, Atabak Ghanizadeh, Hurt, Andrew P., Elmes, Victoria, Boateng, Joshua ORCID: 0000-0002-6310-729X and Douroumis, Dionysios ORCID: 0000-0002-3782-0091 (2020) Bioprinting and preliminary testing of highly reproducible novel bioink for potential skin regeneration. Pharmaceutics, 12 (6):550. ISSN 1999-4923 (Print), 1999-4923 (Online) (doi:https://doi.org/10.3390/pharmaceutics12060550)

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Abstract

Three-dimensional (3D) bioprinting is considered as a novel approach in biofabricating cell-laden constructs that could potentially be used to promote skin regeneration following injury. In this study, a novel crosslinked chitosan (CH)–genipin (GE) bioink laden with keratinocyte and human dermal fibroblast cells was developed and printed successfully using an extruder-based bioprinter. By altering the composition and degree of CH–GE crosslinking, bioink printability was further assessed and compared with a commercial bioink. Rheological analysis showed that the viscosity of the optimised bioink was in a suitable range that facilitated reproducible and reliable printing by applying low pressures ranging from 20–40 kPa. The application of low printing pressures proved vital for viability of cells loaded within the bioinks. Further characterisation using MTT assay showed that cells were still viable within the printed construct at 93% despite the crosslinking, processing and after subjecting to physiological conditions for seven days. The morphological study of the printed cells showed that they were mobile within the bioink. Furthermore, the multi-layered 3D printed constructs demonstrated excellent self-supportive structures in a consistent manner.

Item Type: Article
Uncontrolled Keywords: 3D bioprinting, bioink, chitosan, genipin, human dermal fibroblasts, primary epidermal keratinocytes, skin regeneration
Subjects: Q Science > Q Science (General)
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Centre for Innovation and Process Engineering in Research (CIPER)
Faculty of Engineering & Science > School of Science (SCI)
Last Modified: 16 Jun 2020 13:41
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
Selected for REF2021: None
URI: http://gala.gre.ac.uk/id/eprint/28559

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