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Surface modification of mobile composition of matter (MCM)-41 type silica nanoparticles for potential oral mucosa vaccine delivery

Surface modification of mobile composition of matter (MCM)-41 type silica nanoparticles for potential oral mucosa vaccine delivery

Boateng, Joshua S. ORCID: 0000-0002-6310-729X and Amin, Muhammad Khairul (2020) Surface modification of mobile composition of matter (MCM)-41 type silica nanoparticles for potential oral mucosa vaccine delivery. Journal of Pharmaceutical Sciences, 109 (7). pp. 2271-2283. ISSN 0022-3549 (Print), 1520-6017 (Online) (doi:https://doi.org/10.1016/j.xphs.2020.03.021)

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Abstract

Development of mobile composition of matter (MCM)-41 silica nanoparticles faces challenges, e.g. surface charge properties, antigen loading efficiency, protecting from enzymes and harsh GIT environment and effective release at target mucosal site. We report the production and characterization of polymer and amine modified MCM-41 type silica nanoparticles for oral antigen delivery and with ovalbumin (OVA) as model antigen. Nanoparticles were characterized by dynamic light scattering (DLS), differential scanning calorimetry, X-ray diffraction, scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), circular dichroism (CD), sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), mucin binding, stability in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) and in vitro OVA release in SGF and SIF. Unmodified nanoparticles size 146nm increased to 175-321nm after modification while modified particles remained intact for more than 3 hrs in SGF and 96 hrs in SIF (DLS and SEM). Mucin binding proved PEG and chitosan modified nanoparticles as potential candidates for mucosal oral delivery. Both showed highest OVA encapsulation at 67% and 73%, and sustained OVA release in SIF (96 hrs) at 65% and 64% respectively. BET results showed that nanopores were not blocked during surface modification. CD and SDS-PAGE showed that OVA conformational structure did not change after release from nanoparticles.

Item Type: Article
Uncontrolled Keywords: chitosan, nanocarrier, ovalbumin, mucoadhesive vaccine delivery, polyethylene glycol, silica surface modification
Subjects: Q Science > QC Physics
Q Science > QD Chemistry
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > School of Science (SCI)
Last Modified: 31 Mar 2021 01:38
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
Selected for REF2021: REF 6
URI: http://gala.gre.ac.uk/id/eprint/27646

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