Skip navigation

Mesoporous silica particles as potential carriers for protein drug delivery: protein immobilisation and the effect of displacer on γ-globulin release

Mesoporous silica particles as potential carriers for protein drug delivery: protein immobilisation and the effect of displacer on γ-globulin release

Ajiboye, Adejumoke Lara, Trivedi, Vivek and Mitchell, John ORCID: 0000-0003-2945-3292 (2020) Mesoporous silica particles as potential carriers for protein drug delivery: protein immobilisation and the effect of displacer on γ-globulin release. Drug Development and Industrial Pharmacy, Drug Development and Industrial Pharmacy, 46 (4). pp. 576-586. ISSN 0363-9045 (Print), 1520-5762 (Online) (doi:https://doi.org/10.1080/03639045.2020.1742141)

[img] PDF (Author's Accepted Manuscript)
27425 MITCHELL_Mesoporous_Silica_Particles_As_Potential_Carriers_For_Protein_Drug_Delivery_(AAM)_2020.pdf - Accepted Version
Restricted to Repository staff only until 12 March 2021.

Download (467kB) | Request a copy

Abstract

The adsorption of γ-globulin was evaluated with experiments with silica particles marketed as Syloid AL1-FP (SAL), XDP-3150 (SXDP), and 244FP (SFP). The influence of pH, pore sizes, and degree of surface porosity on the extent of γ-globulin immobilisation was examined. Protein adsorption on these particles was largely related to their surface porosity and pore sizes. The adsorption capacity was established to be greater with mesoporous SFP and SXDP particles at 474 and 377 mg/g respectively when compared to significantly low-porosity SAL (16 mg/g). Additionally, γ-globulin immobilisation was favoured at pH closer to iso-electric point. A key aim of this work was to better understand and improve the limited reversibility of protein adsorption. Protein desorption was found to be lower in simulated intestinal fluid (SIF) in comparison to pH 7.4 phosphate buffer (PB). The use of displacer molecules [sodium dodecyl sulphate (SDS)/tween80/pluronic F127 (PF127)] promoted protein desorption from the adsorbent surface by the exchange mechanism. The PF127 provided substantial release in both studied condition but the highest release of 83% of γ-globulin from SXDP was obtained with tween 80 in PB. The released protein was analysed with circular dichroism (CD) spectroscopy which indicated that the secondary structure of desorbed γ-globulin was dependent on the pH and displacer molecule. The conformation largely remained unchanged when desorption was carried out in SIF but changed markedly in PB specially in the presence of SDS.

Item Type: Article
Uncontrolled Keywords: Mesoporous silica particles, carriers for protein drug delivery, protein immobilisation, γ-globulin release
Subjects: R Medicine > RS Pharmacy and materia medica
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Medway Centre for Pharmaceutical Science
Faculty of Engineering & Science > School of Science (SCI)
Last Modified: 29 May 2020 13:07
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
Selected for REF2021: None
URI: http://gala.gre.ac.uk/id/eprint/27425

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics