Skip navigation

Myristic acid coated protein immobilised mesoporous silica particles as pH induced oral delivery system for the delivery of biomolecules

Myristic acid coated protein immobilised mesoporous silica particles as pH induced oral delivery system for the delivery of biomolecules

Trivedi, Vivek, Bhomia, Ruchir and Mitchell, John ORCID: 0000-0003-2945-3292 (2019) Myristic acid coated protein immobilised mesoporous silica particles as pH induced oral delivery system for the delivery of biomolecules. Pharmaceuticals, 12 (4):153. ISSN 1424-8247 (doi:https://doi.org/10.3390/ph12040153)

[img]
Preview
PDF (Open Access Article)
25603 MITCHELL_Myristic_Acid_Coated_Protein_Immobilised_Mesoporous_Silica_Particles_(OA)_2019.pdf - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

Solid core drug delivery systems (SCDDS) were prepared for the oral delivery of biomolecules using mesoporous silica as core, bovine haemoglobin (bHb) as model drug and supercritical fluid (SCF) processing as encapsulation technique. The use of organic solvents or harsh processing conditions in the development of drug delivery systems for biomolecules can be detrimental for the structural integrity of the molecule. Hence, the coating on protein-immobilised particles was performed via supercritical carbon dioxide (scCO2) processing at a temperature lower than the melting point of myristic acid (MA) to avoid any thermal degradation of bHb. The SCDDS were prepared by bHb immobilisation on mesoporous silica followed by myristic acid (MA) coating at 43 °C and 100 bar in scCO2. bHb-immobilised silica particles were also coated via solvent evaporation (SE) to compare the protein release with scCO2 processed formulations. In both cases, MA coating provided required enteric protection and restricted the bHb release for the first two hours in simulated gastric fluid (SGF). The protein release was immediate upon the change of media to simulated intestinal fluid (SIF), reaching 70% within three hours. The release from SCF processed samples was slower than SE formulations, indicating superior surface coverage of MA on particles in comparison to the SE method. Most importantly, the protein conformation remained unchanged after the release from SCDDS as confirmed by circular dichroism. This study clearly demonstrates that the approach involving protein immobilisation on silica and scCO2 assisted melt-coating method can protect biomolecules from gastric environment and provide the required release of a biologic in intestine without any untoward effects on protein conformation during processing or after release.

Item Type: Article
Uncontrolled Keywords: biomolecules, oral delivery, supercritical CO2, myristic acid, haemoglobin
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
R Medicine > R Medicine (General)
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Medway Centre for Pharmaceutical Science
Faculty of Engineering & Science > School of Science (SCI)
Last Modified: 16 Oct 2019 12:20
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
URI: http://gala.gre.ac.uk/id/eprint/25603

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics