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Transcriptional and metabolomic analyses indicate that cell wall properties are associated with drought tolerance in Brachypodium distachyon

Transcriptional and metabolomic analyses indicate that cell wall properties are associated with drought tolerance in Brachypodium distachyon

Lenk, Ingo ORCID: 0000-0002-9690-1633, Fisher, Lorraine H. C. ORCID: 0000-0002-9690-1633, Vickers, Martin ORCID: 0000-0002-0961-9817, Akinyemi, Aderemi ORCID: 0000-0003-1990-589X, Didion, Thomas, Swain, Martin, Sig Jensen, Christian, Mur, Luis A. J. and Bosch, Maurice (2019) Transcriptional and metabolomic analyses indicate that cell wall properties are associated with drought tolerance in Brachypodium distachyon. International Journal of Molecular Sciences, 20 (7):1758. ISSN 1661-6596 (Print), 1422-0067 (Online) (doi:https://doi.org/10.3390/ijms20071758)

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Abstract

Brachypodium distachyon is an established model for drought tolerance. We previously identified accessions exhibiting high tolerance, susceptibility and intermediate tolerance to drought; respectively, ABR8, KOZ1 and ABR4. Transcriptomics and metabolomic approaches were used to define tolerance mechanisms. Transcriptional analyses suggested relatively few drought responsive genes in ABR8 compared to KOZ1. Linking these to gene ontology (GO) terms indicated enrichment for “regulated stress response”, “plant cell wall” and “oxidative stress” associated genes. Further, tolerance correlated with pre-existing differences in cell wall-associated gene expression including glycoside hydrolases, pectin methylesterases, expansins and a pectin acetylesterase. Metabolomic assessments of the same samples also indicated few significant changes in ABR8 with drought. Instead, pre-existing differences in the cell wall-associated metabolites correlated with drought tolerance. Although other features, e.g., jasmonate signaling were suggested in our study, cell wall-focused events appeared to be predominant. Our data suggests two different modes through which the cell wall could confer drought tolerance: (i) An active response mode linked to stress induced changes in cell wall features, and (ii) an intrinsic mode where innate differences in cell wall composition and architecture are important. Both modes seem to contribute to ABR8 drought tolerance. Identification of the exact mechanisms through which the cell wall confers drought tolerance will be important in order to inform development of drought tolerant crops.

Item Type: Article
Additional Information: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Uncontrolled Keywords: Brachypodium distachyon; cell wall; drought; FIE-MS; mass spectroscopy; metabolite profiling; RNA-seq; transcriptome profiling
Subjects: S Agriculture > S Agriculture (General)
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Natural Resources Institute
Faculty of Engineering & Science > Natural Resources Institute > Food & Markets Department
Last Modified: 28 May 2019 16:36
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: GREAT 2
URI: http://gala.gre.ac.uk/id/eprint/23602

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