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Prediction of human intestinal absorption using micellar liquid chromatography with an aminopropyl stationary phase

Prediction of human intestinal absorption using micellar liquid chromatography with an aminopropyl stationary phase

Shokry, Dina S., Waters, Laura J., Parkes, Gareth M.B. and Mitchell, John C. ORCID: 0000-0003-2945-3292 (2019) Prediction of human intestinal absorption using micellar liquid chromatography with an aminopropyl stationary phase. Biomedical Chromatography:e4515. ISSN 0269-3879 (Print), 1099-0801 (Online) (In Press) (doi:https://doi.org/10.1002/bmc.4515)

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Abstract

The extent of human intestinal absorption (HIA) for a drug is considered to be an important pharmacokinetic parameter which must be determined for orally administered drugs. Traditional experimental methods relied upon animal testing and are renowned for being time consuming, expensive as well as being ethically unfavourable. As a result, developing alternative methods to evaluate a drug's pharmacokinetics is crucial. Micellar liquid chromatography (MLC) is considered to be one of these methods that can replace the use of animals in prediction of HIA. In this study, the combination of an aminopropyl column with the biosurfactant sodium deoxycholate (NaDC) bile salt were used in the experimental determination of micelle-water partition coefficients (log Pmw) for a group of compounds. Multiple linear regression (MLR) was then used for the prediction of HIA using the experimentally determined log Pmw along with other molecular descriptors leading to the construction of a model equation of R2= 85 % and a prediction power represented by R2Pred. =72 %. The use of MLC with an aminopropyl column in combination with NaDC was found to be a good method for the prediction of human intestinal absorption, providing data for a far wider range of compounds compared with previous studies.

Item Type: Article
Additional Information: Unmapped bibliographic data: M3 - doi: 10.1002/bmc.4515 [Field not mapped to EPrints] JA - Biomedical Chromatography [Field not mapped to EPrints] JO - Biomedical Chromatography [Field not mapped to EPrints]
Uncontrolled Keywords: Aminopropyl column, biosurfactant, human intestinal absorption, micellar liquid chromatography, multiple linear regression, pharmacokinetics, sodium deoxycholate
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Medway Centre for Pharmaceutical Science
Faculty of Engineering & Science > Medway Centre for Pharmaceutical Science > Pharmaceutical and Medicinal Science Research Group
Last Modified: 18 Apr 2019 11:54
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: GREAT 1
URI: http://gala.gre.ac.uk/id/eprint/23117

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