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Vertical transmission and early diagnosis of the microsporidian Enterocytozoon hepatonaei in Whiteleg Shrimp Penaeus vannamei

Vertical transmission and early diagnosis of the microsporidian Enterocytozoon hepatonaei in Whiteleg Shrimp Penaeus vannamei

Khac, Hung Vu, Thanh, Thuy Nguyen Thi, Thu, Giang Nguyen Thi, Le, Chi Hieu ORCID: 0000-0002-5168-2297 and Nguyen, Van Duy (2018) Vertical transmission and early diagnosis of the microsporidian Enterocytozoon hepatonaei in Whiteleg Shrimp Penaeus vannamei. Journal of Pure and Applied Microbiology, 12 (3). ISSN 0973-7510 (Print), 2581-690X (Online)

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Abstract

Microsporidia, which are ubiquitous obligate intracellular parasites responsible for a variety of diseases and economic losses in farming, have different transmission strategies. While horizontal transmission relies on sufûcient parasite numbers released into environment, vertical transmission requires host reproduction to occur leading to a lower virulence, and also induces a sex ratio distortion. The second strategy has been reported in a broad range of hosts from protists to mammals, in which insects and amphipod crustaceans as the most common. The present study shows the first evidence of vertical transmission of a microsporidia in decapod crustaceans via an experimental description of the infection by Enterocytozoon hepatopenaei (EHP) in whiteleg shrimp Penaeus vannamei. The group of healthy shrimp was infected with EHP by feeding with infected-shrimp tissue and sharing habitat. The presence of EHP in the infected shrimps was detected by using Polymerase Chain Reaction (PCR) method with specific primers EHP-510F/EHP-510R and histopathological analysis. The Nauplius, Zoae 1 and Zoae 2 stages collected from the infected female broodstocks demonstrated that EHP can infect offspring from their parental shrimp, and interestingly EHP can be detected from Nauplius stage of shrimp by the use of PCR method.

Item Type: Article
Additional Information: This work is licensed under a Creative Commons Attribution 4.0 International License.
Uncontrolled Keywords: aquaculture, diagnosis, Enterocytozoon hepatopenaei (EHP), PCR (Polymerase Chain Reaction), pathogenesis, shrimp.
Subjects: T Technology > TA Engineering (General). Civil engineering (General)
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Department of Engineering Science
Last Modified: 19 May 2019 23:30
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: GREAT 4
URI: http://gala.gre.ac.uk/id/eprint/22478

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