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Cryopreservation of quince (Cydonia oblonga mill.)

Cryopreservation of quince (Cydonia oblonga mill.)

Mehra, Aradhana (2014) Cryopreservation of quince (Cydonia oblonga mill.). CryoLetters, 35 (3). pp. 188-196.

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Abstract

BACKGROUND:
Quince (Cydonia oblonga Mill.) has great potential for utilisation in pharmaceutical and food industries.

OBJECTIVE:
The study was to develop an efficient cryopreservation approach for quince.

METHODS:
Factors on the survival and regrowth such as cold acclimation, explant type and recovery media composition were assessed. The effectiveness of the resultant protocols for a number of quince cultivars was determined.

RESULTS and CONCLUSION:
Quince shoot tips and nodal sections are successfully cryopreserved. Sustained regrowth of quince ‘Angers A’ was observed after encapsulation-osmoprotection/dehydration, encapsulation-dehydration and PVS2 vitrification. The highest regrowth rate (80%) was obtained from explants excised from cold hardened shoots and cryopreserved using encapsulation osmoprotection/dehydration and vitrification protocols. The optimised vitrification protocol in combination with shoot cold hardening and a MS recovery mediumwithout activated charcoal and auxin resulted in satisfactory regrowth of shoots from six quince cultivars. The morphology of acclimatised plants derived from cryopreserved shoots was comparable with non-cryopreserved plants.

Item Type: Article
Uncontrolled Keywords: quince, shoot tips, nodal sections, cold hardening, recovery medium
Faculty / Department / Research Group: Research and Enterprise Training Unit (RETI)
Last Modified: 13 Apr 2018 16:37
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
URI: http://gala.gre.ac.uk/id/eprint/19545

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