Skip navigation

Potential of new isolates of Dunaliella Salina for natural β-Carotene production

Potential of new isolates of Dunaliella Salina for natural β-Carotene production

Xu, Yanan ORCID: 0000-0002-1697-6070, Ibrahim, Iskander M., Wosu, Chiziezi I., Ben-Amotz, Ami and Harvey, Patricia J. ORCID: 0000-0001-7193-4570 (2018) Potential of new isolates of Dunaliella Salina for natural β-Carotene production. Biology, 7 (1). p. 14. ISSN 2079-7737 (Print), 2079-7737 (Online) (doi:https://doi.org/10.3390/biology7010014)

[img]
Preview
PDF (Publisher's PDF - Open Access)
19141 HARVEY_ Potential_of New_Isolates_of_Dunaliella_Salina_2018.pdf - Published Version
Available under License Creative Commons Attribution.

Download (5MB) | Preview

Abstract

The halotolerant microalga Dunaliella salina has been widely studied for natural β-carotene production. This work shows biochemical characterization of three newly isolated Dunaliella salina strains, DF15, DF17, and DF40, compared with D. salina CCAP 19/30 and D. salina UTEX 2538 (also known as D. bardawil). Although all three new strains have been genetically characterized as Dunaliella salina strains, their ability to accumulate carotenoids and their capacity for photoprotection against high light stress are different. DF15 and UTEX 2538 reveal great potential for producing a large amount of β-carotene and maintained a high rate of photosynthesis under light of high intensity; however, DF17, DF40, and CCAP 19/30 showed increasing photoinhibition with increasing light intensity, and reduced contents of carotenoids, in particular β-carotene, suggesting that the capacity of photoprotection is dependent on the cellular content of carotenoids, in particular β-carotene. Strong positive correlations were found between the cellular content of all-trans β-carotene, 9-cis β-carotene, all-trans α-carotene and zeaxanthin but not lutein in the D. salina strains. Lutein was strongly correlated with respiration in photosynthetic cells and strongly related to photosynthesis, chlorophyll and respiration, suggesting an important and not hitherto identified role for lutein in coordinated control of the cellular functions of photosynthesis and respiration in response to changes in light conditions, which is broadly conserved in Dunaliella strains. Statistical analysis based on biochemical data revealed a different grouping strategy from the genetic classification of the strains. The significance of these data for strain selection for commercial carotenoid production is discussed.

Item Type: Article
Additional Information: © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Uncontrolled Keywords: Dunaliella salina; new isolates; characterization; light intensity; β-carotene; carotenoids; correlations; lutein; classification
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 20 May 2019 07:14
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: GREAT b
Selected for GREAT 2019: GREAT 3
URI: http://gala.gre.ac.uk/id/eprint/19141

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics