Skip navigation

High-content imaging of presynaptic assembly

High-content imaging of presynaptic assembly

Poon, Vivian Y., Goh, Chiatzun, Voorhoeve, P. Mathijs and Fivaz, Marc ORCID: 0000-0003-1003-7934 (2014) High-content imaging of presynaptic assembly. Frontiers in Cellular Neuroscience, 8. ISSN 1662-5102 (Online) (doi:https://doi.org/10.3389/fncel.2014.00066)

[img]
Preview
PDF (Publisher's PDF - Open Access)
17891 FIVAZ_High-Content_Imaging_2014.pdf - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

Presynaptic assembly involves the specialization of a patch of axonal membrane into a complex structure that supports synaptic vesicle exocytosis and neurotransmitter release. In mammalian neurons, presynaptic assembly is widely studied in a co-culture assay, where a synaptogenic cue expressed at the surface of a heterologous cell induces presynaptic differentiation in a contacting axon. This assay has led to the discovery of numerous synaptogenic proteins, but has not been used to probe neuronal mechanisms regulating presynaptic induction. The identification of regulatory pathways that fine-tune presynaptic assembly is hindered by the lack of adequate tools to quantitatively image this process. Here, we introduce an image-processing algorithm that identifies presynaptic clusters in mammalian co-cultures and extracts a range of synapse-specific parameters. Using this software, we assessed the intrinsic variability of this synaptic induction assay and probed the effect of eight neuronal microRNAs on presynaptic assembly. Our analysis revealed a novel role for miR-27b in augmenting the density of presynaptic clusters. Our software is applicable to a wide range of synaptic induction protocols (including spontaneous synaptogenesis observed in neuron cultures) and is a valuable tool to determine the subtle impact of disease-associated genes on presynaptic assembly.

Item Type: Article
Additional Information: Copyright © 2014 Poon, Goh, Voorhoeve and Fivaz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Uncontrolled Keywords: Image processing; microRNA (miRNA); Presynaptic terminals; Primary neuron culture; Synapse formation; Synaptic vesicles; Synaptogenic proteins
Faculty / School / Research Centre / Research Group: Faculty of Engineering & Science
Faculty of Education, Health & Human Sciences > School of Human Sciences (HUM)
Last Modified: 09 Oct 2021 04:46
URI: http://gala.gre.ac.uk/id/eprint/17891

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics