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Impaired spatial memory and enhanced long-term potentiation in mice with forebrain-specific ablation of the Stim genes

Impaired spatial memory and enhanced long-term potentiation in mice with forebrain-specific ablation of the Stim genes

Garcia-Alvarez, Gisela, Shetty, Mahesh S., Lu, Bo, Yap, Kenrick An Fu, Oh-Hora, Masatsugu, Sajikumar, Sreedharan, Bichler, Zoë and Fivaz, Marc ORCID: 0000-0003-1003-7934 (2015) Impaired spatial memory and enhanced long-term potentiation in mice with forebrain-specific ablation of the Stim genes. Frontiers in Behavioral Neuroscience, 9:180. ISSN 1662-5153 (Online) (doi:https://doi.org/10.3389/fnbeh.2015.00180)

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Abstract

Recent findings point to a central role of the endoplasmic reticulum-resident STIM (Stromal Interaction Molecule) proteins in shaping the structure and function of excitatory synapses in the mammalian brain. The impact of the Stim genes on cognitive functions remains, however, poorly understood. To explore the function of the Stim genes in learning and memory, we generated three mouse strains with conditional deletion (cKO) of Stim1 and/or Stim2 in the forebrain. Stim1, Stim2, and double Stim1/Stim2 cKO mice show no obvious brain structural defects or locomotor impairment. Analysis of spatial reference memory in the Morris water maze revealed a mild learning delay in Stim1 cKO mice, while learning and memory in Stim2 cKO mice was indistinguishable from their control littermates. Deletion of both Stim genes in the forebrain resulted, however, in a pronounced impairment in spatial learning and memory reflecting a synergistic effect of the Stim genes on the underlying neural circuits. Notably, long-term potentiation (LTP) at CA3-CA1 hippocampal synapses was markedly enhanced in Stim1/Stim2 cKO mice and was associated with increased phosphorylation of the AMPA receptor subunit GluA1, the transcriptional regulator CREB and the L-type Voltage-dependent Ca(2+) channel Cav1.2 on protein kinase A (PKA) sites. We conclude that STIM1 and STIM2 are key regulators of PKA signaling and synaptic plasticity in neural circuits encoding spatial memory. Our findings also reveal an inverse correlation between LTP and spatial learning/memory and suggest that abnormal enhancement of cAMP/PKA signaling and synaptic efficacy disrupts the formation of new memories.

Item Type: Article
Additional Information: Copyright © 2015 Garcia-Alvarez, Shetty, Lu, Yap, Oh-Hora, Sajikumar, Bichler and Fivaz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Uncontrolled Keywords: AMPA receptor; PKA; STIM1 and STIM2; endoplasmic reticulum; excitatory synapse; long-term potentiation; spatial memory
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 16 May 2019 12:04
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: GREAT b
Selected for GREAT 2019: GREAT 2
URI: http://gala.gre.ac.uk/id/eprint/17887

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