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The bacterial communities in plant phloem-sap-feeding insects

The bacterial communities in plant phloem-sap-feeding insects

Jing, Xiangfeng, Wong, Adam C-N, Chaston, John M., Colvin, John, McKenzie, Cindy L. and Douglas, Angela E. (2014) The bacterial communities in plant phloem-sap-feeding insects. Molecular Ecology, 23 (6). pp. 1433-1444. ISSN 0962-1083 (Print), 1365-294X (Online) (doi:https://doi.org/10.1111/mec.12637)

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Abstract

The resident microbiota of animals represents an important contribution to the global microbial diversity, but it is poorly known in many animals. This study investigated the bacterial diversity in plant phloem-sap-feeding whiteflies, aphids and psyllids by pyrosequencing bacterial 16S rRNA gene amplicons. After correction for sequencing error, just 3–7 bacterial operational taxonomic units were recovered from each insect sample sequenced to sufficient depth for saturation of rarefaction curves. Most samples were dominated by primary and secondary symbionts, which are localized to insect cells or the body cavity, indicative of a dearth of bacterial colonists of the gut lumen. Diversity indices of the bacterial communities (Shannon’s index: 0.40–1.46, Simpson’s index: 0.15–0.74) did not differ significantly between laboratory and field samples of the phloem-feeding insects, but were significantly lower than in drosophilid flies quantified by the same methods. Both the low bacterial content of the phloem sap diet and biological processes in the insect may contribute to the apparently low bacterial diversity in these phloem-feeding insects.

Item Type: Article
Uncontrolled Keywords: Acyrthosiphon pisum, bacterial diversity, Bactericera cockerelli, Bemisia tabaci, Diaphorina citri, microbiota, phloem sap, secondary symbiont, symbiosis
Subjects: S Agriculture > SB Plant culture
Faculty / Department / Research Group: Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Last Modified: 10 Jun 2019 15:06
Selected for GREAT 2016: GREAT a
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: GREAT 1
URI: http://gala.gre.ac.uk/id/eprint/15302

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