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Characterization of brown streak virus-resistant cassava

Characterization of brown streak virus-resistant cassava

Maruthi, M. N. ORCID: 0000-0002-8060-866X, Anjanappa, Ravi B., Mehta, Devang, Kanju, Edward, Gruissem, Wilhelm and Vanderschuren, Hervé (2016) Characterization of brown streak virus-resistant cassava. Molecular Plant Microbe Interactions, 29 (7). pp. 527-534. ISSN 0894-0282 (doi:https://doi.org/10.1094/MPMI-01-16-0027-R)

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Abstract

Cassava brown streak disease (CBSD) has become a major constraint to cassava production in East and Central Africa. The identification of new sources of CBSD resistance is essential to deploy CBSD mitigation strategies as the disease is progressing westwards to new geographical areas. A stringent infection method based on top cleft grafting combined with precise virus titer quantitation was utilized to screen fourteen cassava cultivars and elite breeding lines. When inoculated with mixed infections of Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV), the scions of elite breeding lines KBH 2006/18 and KBH 2006/26 remained symptom-free during a 16-week period of virus graft inoculation, while susceptible varieties displayed typical CBSD infection symptoms at 4 weeks after grafting. The identified CBSD resistance was stable under the co-inoculation of CBSV, UCBSV with cassava geminiviruses (CGMs). Double grafting experiments revealed that transmission of CBSV and UCBSV to CBSD susceptible top scions was delayed when using intermediate scions of elite breeding lines KBH 2006/18 and KBH 2006/26. Nonetheless, comparison of virus systemic movement using scions from KBH2006/18 and a transgenic CBSD resistant 60444 line (60444-Hp9 line) showed that both CBSV and UCBSV move at undetectable levels through the stems. Further, protoplast-based assays of virus titers over time showed that the replication of CBSVs is inhibited in the resistant line KBH2006/18, suggesting that the identified CBSD resistance is at least partially based on inhibition of virus replication. Our molecular characterization of CBSD resistance in cassava offers a robust virus–host system to further investigate the molecular determinants of CBSD resistance.

Item Type: Article
Additional Information: Open Access.
Uncontrolled Keywords: Disease resistance, Tropical crop, Cassava, CBSD, CMD, Virus
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Natural Resources Institute
Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Last Modified: 26 Apr 2018 11:16
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: GREAT c
Selected for GREAT 2019: None
URI: http://gala.gre.ac.uk/id/eprint/15163

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