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Molecular characterization of banana bunchy top virus isolate from Sri Lanka and its genetic relationship with other isolates

Molecular characterization of banana bunchy top virus isolate from Sri Lanka and its genetic relationship with other isolates

Wickramaarachchi, W. A. R. T., Shankarappa, K. S., Rangaswamy, K. T., Maruthi, M.N. ORCID: 0000-0002-8060-866X, Rajapakse, R. G. A. S. and Ghosh, Saptarshi (2016) Molecular characterization of banana bunchy top virus isolate from Sri Lanka and its genetic relationship with other isolates. VirusDisease, 27 (2). pp. 154-160. ISSN 2347-3584 (Print), 2347-3517 (Online) (doi:https://doi.org/10.1007/s13337-016-0311-2)

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Abstract

Bunchy top disease of banana caused by Banana bunchy top virus (BBTV, genus Babuvirus family Nanoviridae) is one of the most important constraints in production of banana in the different parts of the world. Six genomic DNA components of BBTV isolate from Kandy, Sri Lanka (BBTV-K) were amplified by polymerase chain reaction (PCR) with specific primers using total DNA extracted from banana tissues showing typical symptoms of bunchy top disease. The amplicons were of expected size of 1.0–1.1 kb, which were cloned and sequenced. Analysis of sequence data revealed the presence of six DNA components; DNA-R, DNA-U3, DNA-S, DNA-N, DNA-M and DNA-C for Sri Lanka isolate. Comparisons of sequence data of DNA components followed by the phylogenetic analysis, grouped Sri Lanka-(Kandy) isolate in the Pacific Indian Oceans (PIO) group. Sri Lanka-(Kandy) isolate of BBTV is classified a new member of PIO group based on analysis of six components of the virus.

Item Type: Article
Uncontrolled Keywords: Banana; Sri Lanka; BBTV; Polymerase; C hain reaction; Sequence; Phylogenetic tree
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Natural Resources Institute
Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Last Modified: 15 Nov 2017 11:08
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/15153

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