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Secondary kinetic isotope effects as probes of environmentally-coupled enzymatic hydrogen tunneling reactions

Secondary kinetic isotope effects as probes of environmentally-coupled enzymatic hydrogen tunneling reactions

Hay, Sam, Pang, Jiayun ORCID: 0000-0003-0689-8440, Monaghan, Phillip J., Wang, Xi, Evans, Rhiannon M., Sutcliffe, Michael J., Allemann, Rudolf K. and Scrutton, Nigel S. (2008) Secondary kinetic isotope effects as probes of environmentally-coupled enzymatic hydrogen tunneling reactions. ChemPhysChem, 9 (11). pp. 1536-1539. ISSN 1439-4235 (Print), 1439-7641 (Online) (doi:https://doi.org/10.1002/cphc.200800291)

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Abstract

The secondary kinetic isotope effect for hydride transfer from NADPH to dihydrofolate catalyzed by dihydrofolate reductase (see traces) is neither temperature dependent nor exalted. In environmentally coupled models of H-tunneling, the secondary isotope effects do not report on promoting motions, but reflect the active site geometry attained immediately prior to H transfer (i.e. the ‘tunnelling ready configuration′).

Item Type: Article
Additional Information: [1] Journal published on behalf of ChemPubSoc Europe.
Uncontrolled Keywords: secondary kinetic isotope effect, hydrogen transfer, dihydrofolate reductase
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Faculty / School / Research Centre / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > School of Science (SCI)
Related URLs:
Last Modified: 15 Nov 2021 23:47
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
Selected for REF2021: None
URI: http://gala.gre.ac.uk/id/eprint/10616

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