TY  - INPR
TI  - Impact of a short?term Nitrate and Citrulline co?supplementation on endurance performance in recreationally trained men and women: a randomized, double?blind, placebo?controlled crossover trial
AV  - public
UR  - https://gala.gre.ac.uk/id/eprint/54629/
JF  - Applied Physiology, Nutrition, and Metabolism
ID  - greenwich54629
SN  - 1715-5312
Y1  - 2026/10/05/
KW  - nutritional supplements
KW  -  lactate
KW  -  energy production
KW  -  Oxide Nitric
KW  -  beetroot
N2  - Dietary nitrate, consumed alone or with citrulline, may enhance endurance performance, but the benefit of co-supplementation in recreationally trained adults remains unclear. This study examined short-term nitrate with and without citrulline for endurance performance and recovery. Twenty-seven adults (17 men, 10 women) completed a randomized, double-blind, placebo-controlled, crossover trial with nitrate (NO??; 600 mg potassium nitrate, ~9.6 mmol), nitrate + citrulline (NO??+CIT; same nitrate + 6 g citrulline-malate), and placebo (maltodextrin). Each condition comprised 4 days supplementation and a 72-h washout; supplements were ingested 2.5 h pre-testing. On day 4, participants completed a graded treadmill test (PGT) to determine peak oxygen uptake (V?O?peak) and maximal aerobic speed (MAS), followed by an all-out run to exhaustion at MAS (All-Out-T). Lactate was measured pre-/post-PGT, 15-min post-PGT, and post-All-Out-T. Outcomes were analysed using linear mixed-effects models. Compared with PLA, V?O?peak increased by 2.6 mL·kg?¹·min?¹ with NO?? (95% CI 0.9 to 4.2; 5.4%, p=0.008) and by 4.8 mL·kg?¹·min?¹ with NO??+CIT (95% CI 3.1 to 6.4; 10.0%, p=0.001). All-Out-T increased by 48 s with NO?? (95% CI 21 to 76; 13.3%, p=0.002) and by 100 s with NO??+CIT (95% CI 73 to 127; 27.4%, p<0.001). NO??+CIT outperformed NO?? for MAS and All-Out-T. Treatment influenced post-exercise lactate concentration and lactate removal, whereas lactate measured 15 min after PGT was unchanged. An exploratory analysis suggested possible Treatment × Sex interactions for V?O?peak and selected lactate outcomes. In conclusion, short-term nitrate improves endurance performance in recreationally trained adults while adding citrulline provides additional benefits for MAS and time-to-exhaustion.
N1  - The project was registered as a clinical trial at the U.S. National Institutes of Health. https://www.clinicaltrials.gov (NCT06531070). Funding was provided jointly by Crown Sport Nutrition and the University of Greenwich. Crown Sport Nutrition provided the study supplements. The sponsor had no role in study design, data collection, data management, statistical analysis, data interpretation, manuscript preparation, or the decision to submit for publication. The University of Greenwich retained full data ownership. The authors report no personal financial relationships  (employment, consultancy, honoraria, advisory roles, equity ownership) with Crown Sport Nutrition.
PB  - Canadian Science Publishing - National Research Council Canada
A1  - Naclerio, Fernando
A1  - Karsten, Bettina
A1  - Larumbe-Zabala, Eneko
ER  -