Hassan, Sally, Colgan, Richard ORCID: 0000-0002-0653-5845, Paul, Mathew J., Atkinson, Christopher J., Sexton, Amy L., van Dolleweerd, Craig J., Keshavarz-Moore, Eli and Ma, Julian K-C. (2012) Recombinant monoclonal antibody yield in transgenic tobacco plants is affected by the wounding response via an ethylene dependent mechanism. Transgenic Research, 21 (6). pp. 1221-1232. ISSN 0962-8819 (Print), 1573-9368 (Online) (doi:https://doi.org/10.1007/s11248-012-9595-1)

Full text not available from this repository.

Abstract

Variability in recombinant IgG yield in transgenic tobacco plants has previously been observed in relation to leaf position, and is interpreted as a function of ageing and the senescence process, leading to increasing protein degradation. Here, similar findings are demonstrated in plants of different ages, expressing IgG but not IgG-HDEL, an antibody form that accumulates within the endoplasmic reticulum. Antibody yields declined following wounding in young transgenic plants expressing IgG but not in those expressing IgG-HDEL. However, in mature IgG plants, the opposite was demonstrated, with significant boosts in yield, while mature IgG-HDEL plants could not be boosted. The lack of response in IgG-HDEL plants suggests that the changes induced by wounding occur post-translationally, and the findings might be explained by wounding responses that differ in plants according to their developmental stages. Plant mechanisms involved in senescence and wounding overlap to a significant degree and compounds such as ethylene, jasmonic acid and salicylic acid are important for mediating downstream effects. Treatment of transgenic plants with ethylene also resulted in a decrease in recombinant IgG yield, which was consistent with the finding that wounded plants could induce lower IgG yields in neighbouring non-wounded plants. Treatment with 1-MCP, an ethylene antagonist, abrogated the IgG yield drop that resulted from wounding, but had no effect on the more gradual IgG yield loss associated with increasing plant age.

Item Type:	Article
Additional Information:	[1] Sally Hassan and Richard Colgan contributed equally to the work. [2] Copyright: (c) Springer Science+Business Media B.V. 2012 [3] Electronic supplementary material The online version of this article (doi:10.1007/s11248-012-9595-1) contains supplementary material, which is available to authorized users.
Uncontrolled Keywords:	nicotiana, transgenic, recombinant protein, 1-MCP, senescence, 27 ethylene, wounding, monoclonal antibody
Subjects:	R Medicine > RS Pharmacy and materia medica S Agriculture > SB Plant culture
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Food & Markets Department
Related URLs:	Publisher
Last Modified:	30 Apr 2020 14:05
URI:	http://gala.gre.ac.uk/id/eprint/8162

Actions (login required)

View Item