Aathithan, Sivasampu (1992) Molecular genetics of 2, 4-dichlorophenoxyacetate (2, 4-D) degradation in pseudomonas cepacia strain 2a. MPhil thesis, Thames Polytechnic.

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Abstract

Pseudomonas cepacia strain 2a, which utilises2,4-dichlorophenoxyacetate (2,4-D) as a sole source of carbon and energy, has been shown to contain an 17kbp plasmid, p2,4-D, which has been mapped using BamHI and Asp718. Several mutants of strain 2a unable to grow on 2,4-D were generated by temperature curing. All bore plasmids of diminished size (57Kbp), created by deletion of 30Kbp from the parental plasmid. These were called p2,4-Del. The plasmid p2,4-Del was presumed to possess the origin of replication and related sequences but had lost some or all of the genes encoding enzymes for 2,4-D degradation.

Item Type:	Thesis (MPhil)
Uncontrolled Keywords:	molecular genetics, biology
Subjects:	Q Science > QH Natural history
Faculty / School / Research Centre / Research Group:	Faculty of Education, Health & Human Sciences > School of Human Sciences (HUM)
Last Modified:	09 Oct 2021 04:46
URI:	http://gala.gre.ac.uk/id/eprint/10334

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