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Preparation and evaluation of streptomycin - and diclofenac-loaded mucoadhesive films for wound healing

Preparation and evaluation of streptomycin - and diclofenac-loaded mucoadhesive films for wound healing

Pawar, H. V., Tetteh, J. and Boateng, Joshua (2012) Preparation and evaluation of streptomycin - and diclofenac-loaded mucoadhesive films for wound healing. In: 22nd Annual Meeting of the Wound Healing Society. 6th Joint Meeting with the European Tissue Repair Society SAWC-Spring/WHS Joint Meeting, April 19-22, 2012, Georgia World Congress Center Atlanta, Georgia, USA.

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Abstract

Solvent cast films comprising synthetic (polyethylene oxide — Polyox™ WSR301 [POL]) and natural (carrageenan [CAR]) polymers together with streptomycin (antimicrobial) combined with diclofenac (anti-inflammatory) for synergisticaction, with the aim of achieving enhanced wound healing effects have been prepared. The films containing different ratios of POL and CAR, plasticized with glycerol were characterized by swelling and hydration capacity, scanning electron microscopy (SEM), differential scanning calorimetry (DSC), texture analysis for mechanical properties and in vitro mucoadhesive properties. In vitro antibacterial studies using disk diffusion test and drug release profiles with Franz diffusion cell were conducted. SEM studies of unplasticized blank films showed presence of spherulitic crystals of POL with homogeneous interpenetrating polymer network. Both blank (BLK) and drug-loaded (DL) films showed homogeneous morphology. DSC results of pure streptomycin and diclofenac showed a melt peak at 146.77 and 293.96°C, respectively; however, this peak was absent in DL films indicating molecular dispersion of the drug. Films containing 25% w/w glycerol exhibited significantly (n = 3, p < 0.001) high work of adhesion, stickiness and cohesiveness with high hydration and swelling index corresponding to unplasticized films. BLK films containing glycerol showed higher tensile strength (TS) (1.1–12.3 MPa) than DLf ilms (0.7–9.5 MPa). Film plasticized with 25% w/w glycerol (1.00 MPa TS, 1031% elongation at break) revealed soft and tough formulation. Antibacterial studies showed that diclofenac and streptomycin significantly (n = 3, p < 0.01)increased zone of inhibition (ZOI) in Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa in contrast to their individual ZOIs. Unplasticized films showed significantly (n = 2 p < 0.0318) higher cumulative percent drug release of streptomycin and diclofenac (60.07 ± 1.56%, 63.39 ± 1.92%, respectively) compared to plasticized films (52.11 ± 1.34%, 55.26 ± 2.25%) within 72 hours. Films prepared from POL and CAR containing streptomycin and diclofenac have potential to prevent and treat wound infections and targets inflammatory phase of wound healing to relieve pain.

Item Type: Conference or Conference Paper (Paper)
Additional Information: Abstract appeared in Wound Repair and Regeneration, Volume 20, Issue 2, pages A12–A48, March-April 2012.
Uncontrolled Keywords: Polyox; Films, Streptomycin; Diclofenac
Subjects: R Medicine > R Medicine (General)
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Department of Pharmaceutical, Chemical & Environmental Sciences
Last Modified: 09 Dec 2016 11:35
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/8266

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