Skip navigation

Metabolic biomarkers of ageing in C57BL/6J wild-type and flavin-containing monooxygenase 5 (FMO5)-knockout mice

Metabolic biomarkers of ageing in C57BL/6J wild-type and flavin-containing monooxygenase 5 (FMO5)-knockout mice

Scott, Flora H., Varshavi, Dorsa, Veeravalli, Sunil, Phillips, Ian R., Veselkov, Kirill, Strittmatter, Nicole, Takats, Zoltan, Shephard, Elizabeth A. and Everett, Jeremy R. ORCID: 0000-0003-1550-4482 (2018) Metabolic biomarkers of ageing in C57BL/6J wild-type and flavin-containing monooxygenase 5 (FMO5)-knockout mice. Frontiers in Molecular Biosciences, 5:28. ISSN 2296-889X (Online) (doi:https://doi.org/10.3389/fmolb.2018.00028)

[img]
Preview
PDF (Publisher's PDF - Open Access)
19766 EVERETT_Metabolic_Biomarkers_of_Ageing_(OA)_2018.pdf - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview
[img]
Preview
PDF (Publisher's PDF Proofs)
19766 EVERETT_Metabolic_Biomarkers_of_Ageing_(Author's_Proof_v.2)_2018.pdf - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview
[img]
Preview
PDF (Supplementary Information)
19766 EVERETT_Supplementary_Information_2018.pdf - Supplemental Material
Available under License Creative Commons Attribution.

Download (4MB) | Preview

Abstract

It was recently demonstrated in mice that knockout of the flavin-containing monooxygenase 5 gene, Fmo5, slows metabolic ageing via pleiotropic effects. We have now used an NMR-based metabonomics approach to study the effects of ageing directly on the metabolic profiles of urine and plasma from male, wild-type C57BL/6J and Fmo5−/− (FMO5 KO) mice back-crossed onto the C57BL/6J background. The aim of this study was to identify metabolic signatures that are associated with ageing in both these mouse lines and to characterize the age-related differences in the metabolite profiles between the FMO5 KO mice and their wild-type counterparts at equivalent time points. We identified a range of age-related biomarkers in both urine and plasma. Some metabolites, including urinary 6-hydroxy-6-methylheptan-3-one (6H6MH3O), a mouse sex pheromone, showed similar patterns of changes with age, regardless of genetic background. Others, however, were altered only in the FMO5 KO, or only in the wild-type mice, indicating the impact of genetic modifications on mouse ageing. Elevated concentrations of urinary taurine represent a distinctive, ageing-related change observed only in wild-type mice.

Item Type: Article
Additional Information: Copyright © 2018 Varshavi, Scott, Varshavi, Veeravalli, Phillips, Veselkov, Strittmatter, Takats, Shephard and Everett. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Uncontrolled Keywords: metabonomics, metabolomics, ageing, C57BL/6J, FMO5 KO, urine, plasma, 6-hydroxy-6-methyl-heptan-3-one
Subjects: Q Science > QD Chemistry
Faculty / School / Research Centre / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > School of Science (SCI)
Related URLs:
Last Modified: 24 Apr 2020 14:29
URI: http://gala.gre.ac.uk/id/eprint/19766

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics