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The influence of mixed solvents volatility on charge state distribution of peptides during positive electrospray ionization mass spectrometry

The influence of mixed solvents volatility on charge state distribution of peptides during positive electrospray ionization mass spectrometry

Nielsen, Birthe V., Abaye, Daniel A. and Nguyen, Minh T. L. (2017) The influence of mixed solvents volatility on charge state distribution of peptides during positive electrospray ionization mass spectrometry. Mass Spectrometry Letters, 8 (2). pp. 29-33. ISSN 2233-4203 (Print), 2093-8950 (Online) (doi:10.5478/MSL.2017.8.2.29)

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Abstract

Understanding the mechanisms that control and concentrate the observed electrospray ionisation (ESI) response from peptides is important. Controlling these mechanisms can improve signal-to-noise ratio in the mass spectrum, and enhances the generation of intact ions, and thus, improves the detection of peptides when analysing mixtures. The effects of different mixtures of aqueous: organic solvents (25, 50, 75%; v/v): formic acid solution (at pH 3.26) compositions on the ESI response and chargestate distribution (CSD) during mass spectrometry (MS) were determined in a group of biologically active peptides (molecular wt range 1.3 - 3.3 kDa). The ESI response is dependent on type of organic solvent in the mobile phase mixture and therefore, solvent choice affects optimal ion intensities. As expected, intact peptide ions gave a more intense ESI signal in polar protic solvent mixtures than in the low polarity solvent. However, for four out of the five analysed peptides, neither the ESI response nor the CSD were affected by the volatility of the solvent mixture. Therefore, in solvent mixtures, as the composition changes during the evaporation processes, the pKb of the amino acid composition is a better predictor of multiple charging of the peptides.

Item Type: Article
Additional Information: All MS Letters content is Open Access, meaning it is accessible online to everyone, without fee and authors’ permission. All MS Letters content is published and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org /licenses/by/3.0/). Under this license, authors reserve the copyright for their content; however, they permit anyone to unrestrictedly use, distribute, and reproduce the content in any medium as far as the original authors and source are cited. For any reuse, redistribution, or reproduction of a work, users must clarify the license terms under which the work was produced.
Uncontrolled Keywords: ESI-MS response, charge-state distribution (CSD), Neuropeptides, Solvent volatility
Subjects: Q Science > QD Chemistry
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Department of Pharmaceutical, Chemical & Environmental Sciences
Last Modified: 17 Sep 2017 21:27
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/17505

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