Bhomia, Ruchir, Trivedi, Vivek ORCID: 0000-0001-9304-9214, Coleman, Nichola J. and Mitchell, John C. ORCID: 0000-0003-2945-3292 (2016) The thermal and storage stability of bovine haemoglobin by ultra violet–visible and circular dichroism spectroscopies. Journal of Pharmaceutical Analysis, 6 (4). pp. 242-248. ISSN 2095-1779 (Print), 2214-0883 (Online) (doi:https://doi.org/10.1016/j.jpha.2016.02.004)

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Abstract

The effects of temperature, pH and long-term storage on the secondary structure and conformation changes of bovine haemoglobin (bHb) were studied using circular dichroism (CD) and ultraviolet-visible (UV-vis) spectroscopies. Neural network software was used to deconvolute the CD data to obtain the fractional content of the five secondary structures. The storage stability of bHb solutions in pH 6, 7 and 8 buffers was significantly higher at 4 °C in comparison to 23 °C for the first 3 days. A complete denaturation of bHb was observed after 40 days irrespective of storage temperature or pH. The bHb solutions were also exposed to heating and cooling cycles between 25 and 65 °C and structural changes were followed by UV-vis and CD spectroscopies. These experiments demonstrated that α-helix content of bHb decreases steadily with increasing temperature above 35 °C at all pH values. The loss in α-helicity and gain in random coil conformations was pH-dependent and greatest under alkaline conditions. Furthermore, there was minimal recovery of the secondary structure content upon cooling to 25 °C. The use of bHb as a model drug is very common and this study illustrates the significance of storage and processing conditions on its stability.

Item Type:	Article
Additional Information:	© 2016 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. This is an open access article under the CCBY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Uncontrolled Keywords:	Bovine haemoglobin, Circular dichroism, Thermal stability, Storage stability
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Education, Health & Human Sciences > School of Human Sciences (HUM)
Last Modified:	09 Oct 2021 04:45
URI:	http://gala.gre.ac.uk/id/eprint/15256

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