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C-reactive protein and complement as acute phase reactants in common carp Cyprinus carpio during CyHV-3 infection

C-reactive protein and complement as acute phase reactants in common carp Cyprinus carpio during CyHV-3 infection

Pionnier, Nicolas, Adamek, Mikolaj, Miest, Joanna J. ORCID: 0000-0003-2534-0530, Harris, Sarah, Matras, Marek, Rakus, Krzysztof Ł., Irnazarow, Ilgiz and Hoole, Dave (2014) C-reactive protein and complement as acute phase reactants in common carp Cyprinus carpio during CyHV-3 infection. Diseases of Aquatic Organisms, 109 (3). pp. 187-199. ISSN 0177-5103 (Print), 1616-1580 (Online) (doi:10.3354/dao02727)

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Abstract

Cyprinid herpesvirus 3 (CyHV-3) is the aetiological agent of a highly virulent and lethal disease of common carp Cyprinus carpio and its ornamental koi varieties. However, specific knowledge about immune mechanisms behind the infection process is very limited. We aimed to evaluate the effect of the CyHV-3 infection on the profile of 2 major components of the common carp immune acute phase response: the C-reactive protein (CRP) and the complement system. Common carp were infected with CyHV-3 by bath immersion. Fish were sampled before the infection and at 6, 12, 24, 72, 120 and 336 h post-infection for serum and head kidney, liver, gill and spleen tissues. CRP levels and complement activity were determined from the serum, whereas CRP- and complement-related genes (crp1, crp2, c1rs, bf/c2, c3, masp2) expression profiles were analysed in the tissues by quantitative PCR. Both CRP levels and complement activity increased significantly up to 10- and 3-fold, respectively, in the serum of infected fish during the challenge. Analysis revealed distinct organ- and time-dependent expression profile patterns for all selected genes. These results suggest that CRP and complement behave as acute phase reactants to CyHV-3 infection in common carp with an organ- and time-dependent response.

Item Type: Article
Additional Information: Information on copyright from the publishers website: "Scientific publications appearing in Inter-Research journals have been rigorously refereed, carefully quality-improved, and professionally selected by our editorial staff. These publications, and all parts thereof, are therefore protected by copyright. This covers the exclusive rights (or the non-exclusive rights in articles issued under the Creative Commons by Attribution (CC-BY) Licence) of the publisher to sell, to reproduce (by any means, including photographic or electronic), to distribute (including via photocopies, reprints, or electronic means), and to store (on microfilm, in electronic data bases, on video disks, etc.) this material. The acceptance regulations of a manuscript for publication automatically include the consent of the author(s) to transfer the copyright to the publisher unless copyright is non-transferable under Crown or U.S. government regulations. Permission for exceptions to these rules on other grounds must be obtained in writing from the publisher at the time of manuscript submission. Copyright in articles issued under the CC-BY Licence remains with the original copyright owners. In the USA, photocopies may be made for personal or in-house use beyond the limitations stipulated under Section 107 or 108 of U.S. Copyright Law."
Uncontrolled Keywords: CRP, Complement, Cyprinid herpesvirus 3, KHV, Koi herpesvirus, Carp, Biomarker
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 17 Oct 2016 09:14
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/15089

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