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Feeding common carp Cyprinus carpio with b-glucan supplemented diet stimulates C-reactive protein and complement immune acute phase responses following PAMPs injection

Feeding common carp Cyprinus carpio with b-glucan supplemented diet stimulates C-reactive protein and complement immune acute phase responses following PAMPs injection

Pionnier, Nicolas, Falco, Alberto, Miest, Joanna J., Shrive, Annette K. and Hoole, Dave (2014) Feeding common carp Cyprinus carpio with b-glucan supplemented diet stimulates C-reactive protein and complement immune acute phase responses following PAMPs injection. Fish & Shellfish Immunology, 39 (2). pp. 285-295. ISSN 1050-4648 (Print), 1095-9947 (Online) (doi:10.1016/j.fsi.2014.05.008)

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Abstract

The effect of β-glucan as a feed additive on the serum and gene profile of C-reactive protein (CRP) and complement acute phase responses was ascertained in common carp Cyprinus carpio. In addition effects of subsequent intraperitoneal injections of pathogen-associated molecular patterns (PAMPs), i.e. LPS or poly(I:C), to mimic bacterial or viral infection respectively, were studied. Carp were first orally fed with β-glucan (MacroGard®) with a daily β-glucan intake of 6 mg per kg body weight or with control food for 25 days and then injected with PBS containing either LPS (4 mg/kg) or poly(I:C) (5 mg/kg) or PBS alone. Fish were sampled during the 25 days of the feeding period and up to 7 days post-PAMPs injections for serum and liver, head kidney and mid-gut tissues. Oral administration of β-glucan for 25 days significantly increased serum CRP levels and alternative complement activity (ACP). In addition, the subsequent LPS and poly(I:C) challenges significantly affected CRP and complement related gene expression profiles (crp1, crp2, c1r/s, bf/c2, c3 and masp2), with the greatest effects observed in the β-glucan fed fish. However, in fish fed β-glucan the PAMPs injections had less effects on CRP levels and complement activity in the serum than in control fed fish, suggesting that the 25 days of β-glucan immunostimulation was sufficient enough to reduce the effects of LPS and poly(I:C) injections. Results suggest that MacroGard® stimulated CRP and complement responses to PAMPs immunological challenges in common carp thus highlighting the beneficial β-glucan immunostimulant properties.

Item Type: Article
Additional Information: © 2014. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords: C-reactive protein Complement b-glucan Immunostimulation PAMPs LPS Poly(I:C) Cyprinus carpio
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 17 Oct 2016 09:14
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/15087

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