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Feeding common carp Cyprinus carpio with b-glucan supplemented diet stimulates C-reactive protein and complement immune acute phase responses following PAMPs injection

Feeding common carp Cyprinus carpio with b-glucan supplemented diet stimulates C-reactive protein and complement immune acute phase responses following PAMPs injection

Pionnier, Nicolas, Falco, Alberto, Miest, Joanna J. ORCID: 0000-0003-2534-0530, Shrive, Annette K. and Hoole, Dave (2014) Feeding common carp Cyprinus carpio with b-glucan supplemented diet stimulates C-reactive protein and complement immune acute phase responses following PAMPs injection. Fish & Shellfish Immunology, 39 (2). pp. 285-295. ISSN 1050-4648 (Print), 1095-9947 (Online) (doi:10.1016/j.fsi.2014.05.008)

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Abstract

The effect of β-glucan as a feed additive on the serum and gene profile of C-reactive protein (CRP) and complement acute phase responses was ascertained in common carp Cyprinus carpio. In addition effects of subsequent intraperitoneal injections of pathogen-associated molecular patterns (PAMPs), i.e. LPS or poly(I:C), to mimic bacterial or viral infection respectively, were studied. Carp were first orally fed with β-glucan (MacroGard®) with a daily β-glucan intake of 6 mg per kg body weight or with control food for 25 days and then injected with PBS containing either LPS (4 mg/kg) or poly(I:C) (5 mg/kg) or PBS alone. Fish were sampled during the 25 days of the feeding period and up to 7 days post-PAMPs injections for serum and liver, head kidney and mid-gut tissues. Oral administration of β-glucan for 25 days significantly increased serum CRP levels and alternative complement activity (ACP). In addition, the subsequent LPS and poly(I:C) challenges significantly affected CRP and complement related gene expression profiles (crp1, crp2, c1r/s, bf/c2, c3 and masp2), with the greatest effects observed in the β-glucan fed fish. However, in fish fed β-glucan the PAMPs injections had less effects on CRP levels and complement activity in the serum than in control fed fish, suggesting that the 25 days of β-glucan immunostimulation was sufficient enough to reduce the effects of LPS and poly(I:C) injections. Results suggest that MacroGard® stimulated CRP and complement responses to PAMPs immunological challenges in common carp thus highlighting the beneficial β-glucan immunostimulant properties.

Item Type: Article
Additional Information: © 2014. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords: C-reactive protein Complement b-glucan Immunostimulation PAMPs LPS Poly(I:C) Cyprinus carpio
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 17 Oct 2016 09:14
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/15087

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