Skip navigation

Time and concentration dependency of MacroGard® induced apoptosis

Time and concentration dependency of MacroGard® induced apoptosis

Miest, J. J. ORCID: 0000-0003-2534-0530 and Hoole, D. (2015) Time and concentration dependency of MacroGard® induced apoptosis. Fish & Shellfish Immunology, 42 (2). pp. 363-366. ISSN 1050-4648 (Print), 1095-9947 (Online) (doi:10.1016/j.fsi.2014.11.009)

[img]
Preview
PDF (Author's Accepted Manuscript)
15086_Miest_Time and concentration dependency (AAM) 2015.pdf - Accepted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (186kB) | Preview

Abstract

In previous studies an effect of β-glucan on apoptosis in fish was noted and in this investigation we determine the time and concentration dependency of this effect. Primary cell cultures of pronephric carp cells were incubated for 6, 24, 48 h with various concentrations ranging from 0 to 1000 μg/ml of MacroGard® β-glucan. Apoptosis was monitored via acridine orange staining. Results indicate a clear effect of time and concentration on the induction of apoptosis in vitro, with only concentration ≥500 μg/ml causing significantly higher percentages of apoptotic cells. Apoptosis was detected after 6 h. This concentration dependent effect has to be considered when studying apoptosis in relation to immunostimulation.

Item Type: Article
Additional Information: © 2014. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords: Apoptosis, Beta-glucan, Carp, Concentration/time dependency
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 20 Oct 2016 15:23
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/15086

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics